Genetic diversity of Alternaria species associated with black point in wheat grains

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Turzhanova , A , Khapilina , O , Tumenbayeva , A , Shevtsov , V , Raiser , O & Kalendar , R 2020 , ' Genetic diversity of Alternaria species associated with black point in wheat grains ' , PeerJ , vol. 8 , no. e9097 , 9097 . https://doi.org/10.7717/peerj.9097

Title: Genetic diversity of Alternaria species associated with black point in wheat grains
Author: Turzhanova, Ainur; Khapilina, Oxana; Tumenbayeva, A; Shevtsov, Vladislav; Raiser, Olesya; Kalendar, Ruslan
Other contributor: University of Helsinki, Department of Agricultural Sciences
Date: 2020-05-05
Language: eng
Number of pages: 19
Belongs to series: PeerJ
ISSN: 2167-8359
DOI: https://doi.org/10.7717/peerj.9097
URI: http://hdl.handle.net/10138/316706
Abstract: The genus Alternaria is a widely distributed major plant pathogen that can act as a saprophyte in plant debris. Fungi of this genus frequently infect cereal crops and cause such diseases as black point and wheat leaf blight, which decrease the yield and quality of cereal products. A total of 25 Alternaria sp. isolates were collected from germ grains of various wheat cultivars from different geographic regions in Kazakhstan. We investigated the genetic relationships of the main Alternaria species related to black point disease of wheat in Kazakhstan, using the inter-primer binding site (iPBS) DNA profiling technique. We used 25 retrotransposon-based iPBS primers to identify the differences among and within Alternaria species populations, and analyzed the variation using clustering (UPGMA) and statistical approaches (AMOVA). Isolates of Alternaria species clustered into two main genetic groups, with species of A.alternata and A.tennuissima forming one cluster, and isolates of A. infectoria forming another. The genetic diversity found using retrotransposon profiles was strongly correlated with geographic data. Overall, the iPBS fingerprinting technique is highly informative and useful for the evaluation of genetic diversity and relationships of Alternaria species.
Subject: 4111 Agronomy
11832 Microbiology and virology
1184 Genetics, developmental biology, physiology
Alternaria
BARLEY
ELEMENTS
Fungi
GENOME EVOLUTION
Genetic diversity
IPBS
IRAP
Molecular marker
PATHOGENS
PHYLOGENY
POPULATIONS
REMAP
RETROTRANSPOSON
Retrotransposon
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