Novel strategies for expansion of tooth epithelial stem cells and ameloblast generation

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http://hdl.handle.net/10138/316911

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Binder , M , Biggs , L C , Kronenberg , M S , Schneider , P , Thesleff , I & Balic , A 2020 , ' Novel strategies for expansion of tooth epithelial stem cells and ameloblast generation ' , Scientific Reports , vol. 10 , no. 1 , 4963 . https://doi.org/10.1038/s41598-020-60708-w

Julkaisun nimi: Novel strategies for expansion of tooth epithelial stem cells and ameloblast generation
Tekijä: Binder, Martin; Biggs, Leah C.; Kronenberg, Mark S.; Schneider, Pascal; Thesleff, Irma; Balic, Anamaria
Muu tekijä: University of Helsinki, Institute of Biotechnology
University of Helsinki, Helsinki Institute of Life Science HiLIFE
University of Helsinki, Institute of Biotechnology
University of Helsinki, Institute of Biotechnology
Päiväys: 2020-03-18
Kieli: eng
Sivumäärä: 11
Kuuluu julkaisusarjaan: Scientific Reports
ISSN: 2045-2322
URI: http://hdl.handle.net/10138/316911
Tiivistelmä: Enamel is secreted by ameloblasts derived from tooth epithelial stem cells (SCs). Humans cannot repair or regenerate enamel, due to early loss of tooth epithelial SCs. Contrarily in the mouse incisors, epithelial SCs are maintained throughout life and endlessly generate ameloblasts, and thus enamel. Here we isolated Sox2-GFP+ tooth epithelial SCs which generated highly cellular spheres following a novel in vitro strategy. This system enabled analysis of SC regulation by various signaling molecules, and supported the stimulatory and inhibitory roles of Shh and Bmp, respectively; providing better insight into the heterogeneity of the SCs. Further, we generated a novel mouse reporter, Enamelin-tdTomato for identification of ameloblasts in live tissues and cells, and used it to demonstrate presence of ameloblasts in the new 3D co-culture system of dental SCs. Collectively, our results provide means of generating 3D tooth epithelium from adult SCs which can be utilized toward future generation of enamel.
Avainsanat: 1184 Genetics, developmental biology, physiology
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