Tannic acid inhibits electrogenic Na+/HCO3- co-transporter activity in embryonic neural stem cell-derived radial glial-like cells

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http://hdl.handle.net/10138/316972

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Nordström , T , Zhenyu , G , Andersson , L C & Åkerman , K E O 2020 , ' Tannic acid inhibits electrogenic Na+/HCO3- co-transporter activity in embryonic neural stem cell-derived radial glial-like cells ' , NeuroReport , vol. 31 , no. 1 , pp. 57-63 . https://doi.org/10.1097/WNR.0000000000001372

Title: Tannic acid inhibits electrogenic Na+/HCO3- co-transporter activity in embryonic neural stem cell-derived radial glial-like cells
Author: Nordström, Tommy; Zhenyu, Gao; Andersson, Leif C.; Åkerman, Karl E. O.
Contributor: University of Helsinki, Medicum
University of Helsinki, Department of Pathology
University of Helsinki, Medicum
Date: 2020-01-08
Language: eng
Number of pages: 7
Belongs to series: NeuroReport
ISSN: 0959-4965
URI: http://hdl.handle.net/10138/316972
Abstract: Self-renewing neural stem cells and progenitor cells are cell populations that generate radial glial cells and neurons through asymmetric division. Regulation of intracellular pH in stem cells with high metabolic activity is critical for both cell signaling and proliferation. We have recently found that a S0859-inhibitable electrogenic Na+/HCO3- co-transporter (NBCe1, Slc4a4), is the primary pH(i) regulatory mechanism in stem cell-derived radial glial-like cells. Here we show, by using the voltage-sensitive fluorescent dye DiBAC 4(3) and BCECF, a pH-sensitive dye, that an antioxidant, tannic acid (100 mu M), can inhibit potassium- and calcium-dependent rapid changes in membrane potential and NBCe1 mediated pH i regulation in brain-derived glial-like cells in vitro. Furthermore, neural stem cell differentiation and neurosphere formation (proliferation) were completely inhibited by tannic acid. The present study provides evidence that tannic acid is a natural inhibitor of NBCe1. It is tempting to speculate that tannic acid or related compounds that inhibits NBCe1-mediated pH(i) regulation in glial-like cells may also have bearing on the treatment of glial neoplasms. NeuroReport 31: 57-63 Copyright (c) 2019 The Author(s). Published by Wolters Kluwer Health, Inc.
Subject: 3112 Neurosciences
3124 Neurology and psychiatry
calcium
hyperpolarization
Na+/HCO3− co-transporter
NBCe1
pH
stem cell
tannic acid
1184 Genetics, developmental biology, physiology
calcium
hyperpolarization
Na+/HCO3-co-transporter
NBCe1
pH
stem cell
tannic acid
CHANNELS
EXPRESSION
CANCER
PROLIFERATION
ARREST
HEALTH
BRAIN
PH
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