Differential role of MAX2 and strigolactones in pathogen, ozone, and stomatal responses

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Kalliola , M , Jakobson , L , Davidsson , P , Pennanen , V , Waszczak , C , Yarmolinsky , D , Zamora , O , Palva , E T , Kariola , T , Kollist , H & Brosché , M 2020 , ' Differential role of MAX2 and strigolactones in pathogen, ozone, and stomatal responses ' , Plant Direct , vol. 4 , no. 2 , 00206 . https://doi.org/10.1002/pld3.206

Title: Differential role of MAX2 and strigolactones in pathogen, ozone, and stomatal responses
Author: Kalliola, Maria; Jakobson, Liina; Davidsson, Pär; Pennanen, Ville; Waszczak, Cezary; Yarmolinsky, Dmitry; Zamora, Olena; Palva, E. Tapio; Kariola, Tarja; Kollist, Hannes; Brosché, Mikael
Contributor organization: Faculty of Biological and Environmental Sciences
Viikki Plant Science Centre (ViPS)
Organismal and Evolutionary Biology Research Programme
Plant ROS-Signalling
Molecular and Integrative Biosciences Research Programme
University of Helsinki LumA Science Education Centre
Plant stress and natural variation
Plant Biology
Date: 2020-02-28
Language: eng
Number of pages: 14
Belongs to series: Plant Direct
ISSN: 2475-0328
DOI: https://doi.org/10.1002/pld3.206
URI: http://hdl.handle.net/10138/317038
Abstract: Strigolactones are a group of phytohormones that control developmental processes including shoot branching and various plant-environment interactions in plants. We previously showed that the strigolactone perception mutant more axillary branches 2 (max2) has increased susceptibility to plant pathogenic bacteria. Here we show that both strigolactone biosynthesis (max3 and max4) and perception mutants (max2 and dwarf14) are significantly more sensitive to Pseudomonas syringae DC3000. Moreover, in response to P. syringae infection, high levels of SA accumulated in max2 and this mutant was ozone sensitive. Further analysis of gene expression revealed no major role for strigolactone in regulation of defense gene expression. In contrast, guard cell function was clearly impaired in max2 and depending on the assay used, also in max3, max4, and d14 mutants. We analyzed stomatal responses to stimuli that cause stomatal closure. While the response to abscisic acid (ABA) was not impaired in any of the mutants, the response to darkness and high CO2 was impaired in max2 and d14-1 mutants, and to CO2 also in strigolactone synthesis (max3, max4) mutants. To position the role of MAX2 in the guard cell signaling network, max2 was crossed with mutants defective in ABA biosynthesis or signaling. This revealed that MAX2 acts in a signaling pathway that functions in parallel to the guard cell ABA signaling pathway. We propose that the impaired defense responses of max2 are related to higher stomatal conductance that allows increased entry of bacteria or air pollutants like ozone. Furthermore, as MAX2 appears to act in a specific branch of guard cell signaling (related to CO2 signaling), this protein could be one of the components that allow guard cells to distinguish between different environmental conditions.
Subject: 11831 Plant biology
abscisic acid
CO2 signaling
guard cell signaling
pathogen defense
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion

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