Quantification of Plant Root Species Composition in Peatlands Using FTIR Spectroscopy

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dc.contributor.author Strakova, Petra
dc.contributor.author Larmola, Tuula
dc.contributor.author Andres, Javier
dc.contributor.author Ilola, Noora
dc.contributor.author Launiainen, Piia
dc.contributor.author Edwards, Keith
dc.contributor.author Minkkinen, Kari
dc.contributor.author Laiho, Raija
dc.date.accessioned 2020-07-02T07:00:02Z
dc.date.available 2020-07-02T07:00:02Z
dc.date.issued 2020-05-19
dc.identifier.citation Strakova , P , Larmola , T , Andres , J , Ilola , N , Launiainen , P , Edwards , K , Minkkinen , K & Laiho , R 2020 , ' Quantification of Plant Root Species Composition in Peatlands Using FTIR Spectroscopy ' , Frontiers in plant science , vol. 11 , 597 . https://doi.org/10.3389/fpls.2020.00597
dc.identifier.other PURE: 139940962
dc.identifier.other PURE UUID: df754ec5-aeb5-4440-b9a9-85c3a8990d7b
dc.identifier.other WOS: 000541122100001
dc.identifier.other ORCID: /0000-0001-8520-0084/work/76724138
dc.identifier.uri http://hdl.handle.net/10138/317247
dc.description.abstract Evidence of plant root biomass and production in peatlands at the level of species or plant functional type (PFT) is needed for defining ecosystem functioning and predicting its future development. However, such data are limited due to methodological difficulties and the toilsomeness of separating roots from peat. We developed Fourier transform infrared (FTIR) spectroscopy based calibration models for quantifying the mass proportions of several common peatland species, and alternatively, the PFTs that these species represented, in composite root samples. We further tested whether woody roots could be classified into diameter classes, and whether dead and living roots could be separated. We aimed to solve whether general models applicable in different studies can be developed, and what would be the best way to build such models. FTIR spectra were measured from dried and powdered roots: both "pure roots", original samples of 25 species collected in the field, and "root mixtures", artificial composite samples prepared by mixing known amounts of pure roots of different species. Partial least squares regression was used to build the calibration models. The general applicability of the models was tested using roots collected in different sites or times. Our main finding is that pure roots can replace complex mixtures as calibration data. Using pure roots, we constructed generally applicable models for quantification of roots of the main PFTs of northern peatlands. The models provided accurate estimates even for far distant sites, with root mean square error (RMSE) 1.4-6.6% for graminoids, forbs and ferns. For shrubs and trees the estimates were less accurate due to higher within-species heterogeneity, partly related to variation in root diameter. Still, we obtained RMSE 3.9-10.8% for total woody roots, but up to 20.1% for different woody-root types. Species-level and dead-root models performed well within the calibration dataset but provided unacceptable estimates for independent samples, limiting their routine application in field conditions. Our PFT-level models can be applied on roots separated from soil for biomass determination or from ingrowth cores for estimating root production. We present possibilities for further development of species-level or dead-root models using the pure-root approach. en
dc.format.extent 22
dc.language.iso eng
dc.relation.ispartof Frontiers in plant science
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject FTIR
dc.subject calibration model
dc.subject dead roots
dc.subject fine roots
dc.subject peatland
dc.subject plant root composition
dc.subject plant functional type (PFT)
dc.subject root chemistry
dc.subject WATER-LEVEL DRAWDOWN
dc.subject NET PRIMARY PRODUCTION
dc.subject SOIL ORGANIC-CARBON
dc.subject REFLECTANCE SPECTROSCOPY
dc.subject BOREAL PEATLANDS
dc.subject EUROPEAN BEECH
dc.subject PINE MIRES
dc.subject BIOMASS
dc.subject DYNAMICS
dc.subject LITTER
dc.subject 4112 Forestry
dc.subject 11831 Plant biology
dc.subject 1181 Ecology, evolutionary biology
dc.title Quantification of Plant Root Species Composition in Peatlands Using FTIR Spectroscopy en
dc.type Article
dc.contributor.organization Department of Forest Sciences
dc.contributor.organization Strawberry research group
dc.contributor.organization Department of Agricultural Sciences
dc.contributor.organization Kari Minkkinen / Principal Investigator
dc.contributor.organization Forest Ecology and Management
dc.contributor.organization Plant Production Sciences
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.3389/fpls.2020.00597
dc.relation.issn 1664-462X
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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