A coherent feed-forward loop drives vascular regeneration in damaged aerial organs of plants growing in a normal developmental context

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dc.contributor.author Radhakrishnan, Dhanya
dc.contributor.author Shanmukhan, Anju Pallipurath
dc.contributor.author Kareem, Abdul
dc.contributor.author Aiyaz, Mohammed
dc.contributor.author Varapparambathu, Vijina
dc.contributor.author Toms, Ashna
dc.contributor.author Kerstens, Merijn
dc.contributor.author Valsakumar, Devisree
dc.contributor.author Landge, Amit N.
dc.contributor.author Shaji, Anil
dc.contributor.author Mathew, Mathew K.
dc.contributor.author Sawchuk, Megan G.
dc.contributor.author Scarpella, Enrico
dc.contributor.author Krizek, Beth A.
dc.contributor.author Efroni, Idan
dc.contributor.author Mähönen, Ari Pekka
dc.contributor.author Willemsen, Viola
dc.contributor.author Scheres, Ben
dc.contributor.author Prasad, Kalika
dc.date.accessioned 2020-07-08T13:45:04Z
dc.date.available 2020-07-08T13:45:04Z
dc.date.issued 2020-03
dc.identifier.citation Radhakrishnan , D , Shanmukhan , A P , Kareem , A , Aiyaz , M , Varapparambathu , V , Toms , A , Kerstens , M , Valsakumar , D , Landge , A N , Shaji , A , Mathew , M K , Sawchuk , M G , Scarpella , E , Krizek , B A , Efroni , I , Mähönen , A P , Willemsen , V , Scheres , B & Prasad , K 2020 , ' A coherent feed-forward loop drives vascular regeneration in damaged aerial organs of plants growing in a normal developmental context ' , Development , vol. 147 , no. 6 , 185710 . https://doi.org/10.1242/dev.185710
dc.identifier.other PURE: 140279112
dc.identifier.other PURE UUID: a1d00485-946e-4b18-abde-26ede2669fe9
dc.identifier.other WOS: 000522795700017
dc.identifier.other ORCID: /0000-0001-6051-866X/work/77086493
dc.identifier.uri http://hdl.handle.net/10138/317516
dc.description.abstract Aerial organs of plants, being highly prone to local injuries, require tissue restoration to ensure their survival. However, knowledge of the underlying mechanism is sparse. In this study, we mimicked natural injuries in growing leaves and stems to study the reunion between mechanically disconnected tissues. We show that PLETHORA (PLT) and AINTEGUMENTA (ANT) genes, which encode stem cell-promoting factors, are activated and contribute to vascular regeneration in response to these injuries. PLT proteins bind to and activate the CUC2 promoter. PLT proteins and CUC2 regulate the transcription of the local auxin biosynthesis gene YUC4 in a coherent feed-forward loop, and this process is necessary to drive vascular regeneration. In the absence of this PLT-mediated regeneration response, leaf ground tissue cells can neither acquire the early vascular identity marker ATHB8, nor properly polarise auxin transporters to specify new venation paths. The PLT-CUC2 module is required for vascular regeneration, but is dispensable for midvein formation in leaves. We reveal the mechanisms of vascular regeneration in plants and distinguish between the wound-repair ability of the tissue and its formation during normal development. en
dc.format.extent 13
dc.language.iso eng
dc.relation.ispartof Development
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject Vascular regeneration
dc.subject PLT
dc.subject CUC2
dc.subject Wound repair
dc.subject Auxin
dc.subject PIN1
dc.subject Arabidopsis
dc.subject ARABIDOPSIS
dc.subject TISSUE
dc.subject DIFFERENTIATION
dc.subject PATTERN
dc.subject GENES
dc.subject MODEL
dc.subject ACQUISITION
dc.subject PHYLLOTAXIS
dc.subject EXPRESSION
dc.subject FRAMEWORK
dc.subject 1184 Genetics, developmental biology, physiology
dc.title A coherent feed-forward loop drives vascular regeneration in damaged aerial organs of plants growing in a normal developmental context en
dc.type Article
dc.contributor.organization Helsinki Institute of Life Science HiLIFE
dc.contributor.organization Staff Services
dc.contributor.organization Viikki Plant Science Centre (ViPS)
dc.contributor.organization Plant Biology
dc.contributor.organization Biosciences
dc.contributor.organization Institute of Biotechnology
dc.contributor.organization Faculty of Biological and Environmental Sciences
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1242/dev.185710
dc.relation.issn 0950-1991
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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