B cells rapidly target antigen and surface-derived MHCII into peripheral degradative compartments

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Hernandez-Perez , S , Vainio , M , Kuokkanen , E , Sustar , V , Petrov , P , Forsten , S , Paavola , V , Rajala , J , Awoniyi , L O , Sarapulov , A , Vihinen , H , Jokitalo , E , Bruckbauer , A & Mattila , P K 2020 , ' B cells rapidly target antigen and surface-derived MHCII into peripheral degradative compartments ' , Journal of Cell Science , vol. 133 , no. 5 , 235192 . https://doi.org/10.1242/jcs.235192

Title: B cells rapidly target antigen and surface-derived MHCII into peripheral degradative compartments
Author: Hernandez-Perez, Sara; Vainio, Marika; Kuokkanen, Elina; Sustar, Vid; Petrov, Petar; Forsten, Sofia; Paavola, Vilma; Rajala, Johanna; Awoniyi, Luqman O.; Sarapulov, Alexey; Vihinen, Helena; Jokitalo, Eija; Bruckbauer, Andreas; Mattila, Pieta K.
Contributor: University of Helsinki, Univ Turku, University of Turku, Res Ctr Integrat Physiol & Pharmacol, Inst Biomed
University of Helsinki, Electron Microscopy
University of Helsinki, Electron Microscopy
University of Helsinki, Univ Turku, University of Turku, Res Ctr Integrat Physiol & Pharmacol, Inst Biomed
Date: 2020-03
Language: eng
Number of pages: 15
Belongs to series: Journal of Cell Science
ISSN: 0021-9533
URI: http://hdl.handle.net/10138/317695
Abstract: In order to mount high-affinity antibody responses, B cells internalise specific antigens and process them into peptides loaded onto MHCII for presentation to T helper cells (T H cells). While the biochemical principles of antigen processing and MHCII loading have been well dissected, how the endosomal vesicle system is wired to enable these specific functions remains much less studied. Here, we performed a systematic microscopy-based analysis of antigen trafficking in B cells to reveal its route to the MHCII peptide-loading compartment (MIIC). Surprisingly, we detected fast targeting of internalised antigen into peripheral acidic compartments that possessed the hallmarks of the MIIC and also showed degradative capacity. In these vesicles, intemalised antigen converged rapidly with membrane-derived MHCII and partially overlapped with cathepsin-S and H2-M, both required for peptide loading. These early compartments appeared heterogenous and atypical as they contained a mixture of both early and late endosomal markers, indicating a specialized endosomal route. Together, our data suggest that, in addition to in the previously reported perinuclear late endosomal MIICs, antigen processing and peptide loading could have already started in these specialized early peripheral acidic vesicles (eMlIC) to support fast peptide-MHCII presentation. This article has an associated First Person interview with the first author of the paper.
Subject: Adaptive immune system
B cells
Antigen processing
B cell receptor
BCR
MHCII
Peptide loading
Endosomes
Vesicle traffic
CLASS-II
CUTTING EDGE
HLA-DM
RECEPTOR
PEPTIDE
TRAFFICKING
CYCLOHEXIMIDE
COMPLEXES
VESICLES
LYMPHOCYTES
1182 Biochemistry, cell and molecular biology
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