Comparing serum protein levels can aid in differentiating HPV-negative and -positive oropharyngeal squamous cell carcinoma patients

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dc.contributor.author Dickinson, Amy
dc.contributor.author Saraswat, Mayank
dc.contributor.author Syrjänen, Stina
dc.contributor.author Tohmola, Tiialotta
dc.contributor.author Silén, Robert
dc.contributor.author Randén-Brady, Reija
dc.contributor.author Carpén, Timo
dc.contributor.author Hagström, Jaana
dc.contributor.author Haglund, Caj
dc.contributor.author Mattila, Petri
dc.contributor.author Mäkitie, Antti
dc.contributor.author Joenväärä, Sakari
dc.contributor.author Silén, Suvi
dc.date.accessioned 2020-07-20T10:57:01Z
dc.date.available 2020-07-20T10:57:01Z
dc.date.issued 2020-06-15
dc.identifier.citation Dickinson , A , Saraswat , M , Syrjänen , S , Tohmola , T , Silén , R , Randén-Brady , R , Carpén , T , Hagström , J , Haglund , C , Mattila , P , Mäkitie , A , Joenväärä , S & Silén , S 2020 , ' Comparing serum protein levels can aid in differentiating HPV-negative and -positive oropharyngeal squamous cell carcinoma patients ' , PLoS One , vol. 15 , no. 6 , 0233974 . https://doi.org/10.1371/journal.pone.0233974
dc.identifier.other PURE: 139060143
dc.identifier.other PURE UUID: ec6efed6-77af-4164-897e-e4fbe865c53a
dc.identifier.other RIS: urn:EC3205CE5CE8CB5BB4F66852A7E3BFC0
dc.identifier.other WOS: 000542685100022
dc.identifier.other ORCID: /0000-0001-6079-7881/work/77845305
dc.identifier.uri http://hdl.handle.net/10138/317819
dc.description.abstract Background The surrogate immunohistochemical marker, p16INK4a, is used in clinical practice to determine the high-risk human papillomavirus (HPV) status of oropharyngeal squamous cell carcinomas (OPSCC). With a specificity of 83%, this will misclassify some patients compared with direct HPV testing. Patients who are p16INK4a-positive but HPV DNA-negative, or RNA-negative, may be unsuitable for treatment de-escalation aimed at reducing treatment-related side effects. We aimed to identify cost-effective serum markers to improve decision making for patients at risk of misclassification by p16INK4a alone. Methods Serum proteins from pre-treatment samples of 36 patients with OPSCC were identified and quantified using label-free mass spectrometry-based proteomics. HPV-status was determined using p16INK4a/HPV DNA and E6/E7 mRNA. Serum protein expressions were compared between groups of patients according to HPV status, using the unpaired t-test with a Benjamini-Hochberg correction. ROC curves (AUC) were calculated with SPSS (v25). Results Of 174 serum proteins identified, complement component C7 (C7), apolipoprotein F (ApoF) and galectin-3-Binding Protein (LGALS3BP) significantly differed between HPV-positive and -negative tumors (AUC ranging from 0.84–0.87). ApoF levels were more than twice as high in the E6/E7 mRNA HPV-positive group than HPV-negative. Conclusions Serum C7, ApoF and LGALS3BP levels discriminate between HPV-positive and HPV-negative OPSCC. Further studies are needed to validate these host immunity-related proteins as markers for HPV-associated OPSCC. en
dc.format.extent 13
dc.language.iso eng
dc.relation.ispartof PLoS One
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject 3122 Cancers
dc.subject 3125 Otorhinolaryngology, ophthalmology
dc.subject HUMAN-PAPILLOMAVIRUS
dc.subject DIAGNOSTIC-ACCURACY
dc.subject CANCER
dc.subject HEAD
dc.subject SURVIVAL
dc.subject METASTASIS
dc.subject EXPRESSION
dc.subject DNA
dc.subject C7
dc.subject PREVALENCE
dc.title Comparing serum protein levels can aid in differentiating HPV-negative and -positive oropharyngeal squamous cell carcinoma patients en
dc.type Article
dc.contributor.organization Department of Ophthalmology and Otorhinolaryngology
dc.contributor.organization University of Helsinki
dc.contributor.organization Helsinki University Hospital Area
dc.contributor.organization Research Program in Systems Oncology
dc.contributor.organization Research Programs Unit
dc.contributor.organization Faculty of Medicine
dc.contributor.organization HUSLAB
dc.contributor.organization Transplantation Laboratory
dc.contributor.organization Department of Pathology
dc.contributor.organization Medicum
dc.contributor.organization HUS Head and Neck Center
dc.contributor.organization Department of Surgery
dc.contributor.organization HUS Abdominal Center
dc.contributor.organization II kirurgian klinikka
dc.contributor.organization CAN-PRO - Translational Cancer Medicine Program
dc.contributor.organization Korva-, nenä- ja kurkkutautien klinikka
dc.contributor.organization Clinicum
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1371/journal.pone.0233974
dc.relation.issn 1932-6203
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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