Optimization of a High-Throughput 384-Well Plate-Based Screening Platform with Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 15442 Biofilms

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Gilbert-Girard , S , Savijoki , K , Yli-Kauhaluoma , J & Fallarero , A 2020 , ' Optimization of a High-Throughput 384-Well Plate-Based Screening Platform with Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 15442 Biofilms ' , International Journal of Molecular Sciences , vol. 21 , no. 9 , 3034 . https://doi.org/10.3390/ijms21093034

Title: Optimization of a High-Throughput 384-Well Plate-Based Screening Platform with Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 15442 Biofilms
Author: Gilbert-Girard, Shella; Savijoki, Kirsi; Yli-Kauhaluoma, Jari; Fallarero, Adyary
Other contributor: University of Helsinki, Pharmaceutical Design and Discovery group
University of Helsinki, Division of Pharmaceutical Biosciences
University of Helsinki, Pharmaceutical Design and Discovery group
University of Helsinki, Pharmaceutical Design and Discovery group






Date: 2020-05
Language: eng
Number of pages: 24
Belongs to series: International Journal of Molecular Sciences
ISSN: 1661-6596
DOI: https://doi.org/10.3390/ijms21093034
URI: http://hdl.handle.net/10138/317840
Abstract: In recent years, bacterial infections have become a main concern following the spread of antimicrobial resistance. In addition, bacterial biofilms are known for their high tolerance to antimicrobials and they are regarded as a main cause of recalcitrant infections in humans. Many efforts have been deployed in order to find new antibacterial therapeutic options and the high-throughput screening (HTS) of large libraries of compounds is one of the utilized strategies. However, HTS efforts for anti-biofilm discovery remain uncommon. Here, we miniaturized a 96-well plate (96WP) screening platform, into a 384-well plate (384WP) format, based on a sequential viability and biomass measurements for the assessment of anti-biofilm activity. During the assay optimization process, different parameters were evaluated while using Staphylococcus aureus and Pseudomonas aeruginosa as the bacterial models. We compared the performance of the optimized 384WP platform to our previously established 96WP-based platform by carrying out a pilot screening of 100 compounds, followed by the screening of a library of 2000 compounds to identify new repurposed anti-biofilm agents. Our results show that the optimized 384WP platform is well-suited for screening purposes, allowing for the rapid screening of a higher number of compounds in a run in a reliable manner.
Subject: ANTIBACTERIAL
ASSAY
BACTERIAL-MEMBRANE
IDENTIFICATION
IN-VITRO
INFECTIOUS-DISEASES-SOCIETY
Pseudomonas aeruginosa
QUANTIFICATION
SMALL-MOLECULE INHIBITORS
SUSCEPTIBILITY
Staphylococcus aureus
VALIDATION
bacteria
biofilms
crystal violet
resazurin
screening
116 Chemical sciences
1182 Biochemistry, cell and molecular biology
317 Pharmacy
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