N-glycomic profiling of colorectal cancer according to tumor stage and location

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Holm , M , Nummela , P , Heiskanen , A , Satomaa , T , Kaprio , T , Mustonen , H , Ristimäki , A & Haglund , C 2020 , ' N-glycomic profiling of colorectal cancer according to tumor stage and location ' , PLoS One , vol. 15 , no. 6 , 0234989 . https://doi.org/10.1371/journal.pone.0234989

Title: N-glycomic profiling of colorectal cancer according to tumor stage and location
Author: Holm, Matilda; Nummela, Pirjo; Heiskanen, Annamari; Satomaa, Tero; Kaprio, Tuomas; Mustonen, Harri; Ristimäki, Ari; Haglund, Caj
Contributor organization: Department of Surgery
Department of Pathology
CAN-PRO - Translational Cancer Medicine Program
ATG - Applied Tumor Genomics
Faculty of Medicine
University of Helsinki
Helsinki University Hospital Area
Research Programs Unit
Clinicum
HUS Abdominal Center
HUSLAB
Gastrointestinal tumorigenesis
II kirurgian klinikka
Date: 2020-06-29
Language: eng
Number of pages: 16
Belongs to series: PLoS One
ISSN: 1932-6203
DOI: https://doi.org/10.1371/journal.pone.0234989
URI: http://hdl.handle.net/10138/317847
Abstract: Alterations in glycosylation are seen in many types of cancer, including colorectal cancer (CRC). Glycans, the sugar moieties of glycoconjugates, are involved in many important functions relevant to cancer and can be of value as biomarkers. In this study, we have used mass spectrometry to analyze the N-glycan profiles of 35 CRC tissue samples and 10 healthy tissue samples from non-CRC patients who underwent operations for other reasons. The tumor samples were divided into groups depending on tumor location (right or left colon) and stage (II or III), while the healthy samples were divided into right or left colon. The levels of neutral and acidic N-glycan compositions and glycan classes were analyzed in a total of ten different groups. Surprisingly, there were no significant differences in glycan levels when all right- and left-sided CRC samples were compared, and few differences (such as in the abundance of the neutral N-glycan H3N5) were seen when the samples were divided according to both location and stage. Multiple significant differences were found in the levels of glycans and glycan classes when stage II and III samples were compared, and these glycans could be of value as candidates for new markers of cancer progression. In order to validate our findings, we analyzed healthy tissue samples from the right and left colon and found no significant differences in the levels of any of the glycans analyzed, confirming that our findings when comparing CRC samples from the right and left colon are not due to normal variations in the levels of glycans between the healthy right and left colon. Additionally, the levels of the acidic glycans H4N3F1P1, H5N4F1P1, and S1H5N4F1 were found to change in a cancer-specific but colon location-nonspecific manner, indicating that CRC affects glycan levels in similar ways regardless of tumor location.
Subject: 3122 Cancers
3126 Surgery, anesthesiology, intensive care, radiology
CELL-LINES
GLYCOSYLATION
IDENTIFICATION
BIOMARKERS
PROTEINS
GLYCANS
PLASMA
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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