Holm , M , Nummela , P , Heiskanen , A , Satomaa , T , Kaprio , T , Mustonen , H , Ristimäki , A & Haglund , C 2020 , ' N-glycomic profiling of colorectal cancer according to tumor stage and location ' , PLoS One , vol. 15 , no. 6 , 0234989 . https://doi.org/10.1371/journal.pone.0234989
Title: | N-glycomic profiling of colorectal cancer according to tumor stage and location |
Author: | Holm, Matilda; Nummela, Pirjo; Heiskanen, Annamari; Satomaa, Tero; Kaprio, Tuomas; Mustonen, Harri; Ristimäki, Ari; Haglund, Caj |
Contributor organization: | Department of Surgery Department of Pathology CAN-PRO - Translational Cancer Medicine Program ATG - Applied Tumor Genomics Faculty of Medicine University of Helsinki Helsinki University Hospital Area Research Programs Unit Clinicum HUS Abdominal Center HUSLAB Gastrointestinal tumorigenesis II kirurgian klinikka |
Date: | 2020-06-29 |
Language: | eng |
Number of pages: | 16 |
Belongs to series: | PLoS One |
ISSN: | 1932-6203 |
DOI: | https://doi.org/10.1371/journal.pone.0234989 |
URI: | http://hdl.handle.net/10138/317847 |
Abstract: | Alterations in glycosylation are seen in many types of cancer, including colorectal cancer (CRC). Glycans, the sugar moieties of glycoconjugates, are involved in many important functions relevant to cancer and can be of value as biomarkers. In this study, we have used mass spectrometry to analyze the N-glycan profiles of 35 CRC tissue samples and 10 healthy tissue samples from non-CRC patients who underwent operations for other reasons. The tumor samples were divided into groups depending on tumor location (right or left colon) and stage (II or III), while the healthy samples were divided into right or left colon. The levels of neutral and acidic N-glycan compositions and glycan classes were analyzed in a total of ten different groups. Surprisingly, there were no significant differences in glycan levels when all right- and left-sided CRC samples were compared, and few differences (such as in the abundance of the neutral N-glycan H3N5) were seen when the samples were divided according to both location and stage. Multiple significant differences were found in the levels of glycans and glycan classes when stage II and III samples were compared, and these glycans could be of value as candidates for new markers of cancer progression. In order to validate our findings, we analyzed healthy tissue samples from the right and left colon and found no significant differences in the levels of any of the glycans analyzed, confirming that our findings when comparing CRC samples from the right and left colon are not due to normal variations in the levels of glycans between the healthy right and left colon. Additionally, the levels of the acidic glycans H4N3F1P1, H5N4F1P1, and S1H5N4F1 were found to change in a cancer-specific but colon location-nonspecific manner, indicating that CRC affects glycan levels in similar ways regardless of tumor location. |
Subject: |
3122 Cancers
3126 Surgery, anesthesiology, intensive care, radiology CELL-LINES GLYCOSYLATION IDENTIFICATION BIOMARKERS PROTEINS GLYCANS PLASMA |
Peer reviewed: | Yes |
Rights: | cc_by |
Usage restriction: | openAccess |
Self-archived version: | publishedVersion |
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