Lack of antidepressant effects of burst-suppressing isoflurane anesthesia in adult male Wistar outbred rats subjected to chronic mild stress

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Theilmann , W , Rosenholm , M , Hampel , P , Löscher , W & Rantamäki , T 2020 , ' Lack of antidepressant effects of burst-suppressing isoflurane anesthesia in adult male Wistar outbred rats subjected to chronic mild stress ' , PLoS One , vol. 15 , no. 6 , 0235046 . https://doi.org/10.1371/journal.pone.0235046

Title: Lack of antidepressant effects of burst-suppressing isoflurane anesthesia in adult male Wistar outbred rats subjected to chronic mild stress
Author: Theilmann, Wiebke; Rosenholm, Marko; Hampel, Philip; Löscher, Wolfgang; Rantamäki, Tomi
Contributor: University of Helsinki, INDIVIDRUG - Individualized Drug Therapy
University of Helsinki, Drug Research Program
Date: 2020-06-24
Language: eng
Number of pages: 14
Belongs to series: PLoS One
ISSN: 1932-6203
URI: http://hdl.handle.net/10138/317881
Abstract: Post-ictal emergence of slow wave EEG (electroencephalogram) activity and burst-suppression has been associated with the therapeutic effects of the electroconvulsive therapy (ECT), indicating that mere “cerebral silence” may elicit antidepressant actions. Indeed, brief exposures to burst-suppressing anesthesia has been reported to elicit antidepressant effects in a subset of patients, and produce behavioral and molecular alterations, such as increased expression of brain-derived neurotrophic factor (BDNF), connected with antidepressant responses in rodents. Here, we have further tested the cerebral silence hypothesis by determining whether repeated exposures to isoflurane anesthesia reduce depressive-like symptoms or influence BDNF expression in male Wistar outbred rats (Crl:WI(Han)) subjected to chronic mild stress (CMS), a model which is responsive to repeated electroconvulsive shocks (ECS, a model of ECT). Stress-susceptible, stress-resilient, and unstressed rats were exposed to 5 doses of isoflurane over a 15-day time period, with administrations occurring every third day. Isoflurane dosing is known to reliably produce rapid EEG burst-suppression (4% induction, 2% maintenance; 15 min). Antidepressant and anxiolytic effects of isoflurane were assessed after the first, third, and fifth drug exposure by measuring sucrose consumption, as well as performance on the open field and the elevated plus maze tasks. Tissue samples from the medial prefrontal cortex and hippocampus were collected, and levels of BDNF (brain-derived neurotrophic factor) protein were assessed. We find that isoflurane anesthesia had no impact on the behavior of stress-resilient or anhedonic rats in selected tests; findings which were consistent—perhaps inherently related—with unchanged levels of BDNF.
Subject: 3112 Neurosciences
TRKB NEUROTROPHIN RECEPTOR
RESISTANT MAJOR DEPRESSION
ELECTROCONVULSIVE-THERAPY
MESSENGER-RNAS
AMPA RECEPTOR
BRAIN
BDNF
EXPRESSION
KETAMINE
ACTIVATION
TRKB NEUROTROPHIN RECEPTOR
RESISTANT MAJOR DEPRESSION
ELECTROCONVULSIVE-THERAPY
MESSENGER-RNAS
AMPA RECEPTOR
BRAIN
BDNF
EXPRESSION
KETAMINE
ACTIVATION
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