Untargeted metabolomics analysis of rat hippocampus subjected to sleep fragmentation

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Yoon , D W , Kwon , H N , Jin , X , Kim , J K , Lee , S K , Park , S , Yun , C-H & Shin , C 2019 , ' Untargeted metabolomics analysis of rat hippocampus subjected to sleep fragmentation ' , Brain Research Bulletin , vol. 153 , pp. 74-83 . https://doi.org/10.1016/j.brainresbull.2019.08.008

Title: Untargeted metabolomics analysis of rat hippocampus subjected to sleep fragmentation
Author: Yoon, Dae Wui; Kwon, Hyuk Nam; Jin, Xing; Kim, Jin Kwan; Lee, Seung Ku; Park, Sunghyouk; Yun, Chang-Ho; Shin, Chol
Contributor organization: Kidney development
Helsinki Institute of Life Science HiLIFE, Joint Activities
STEMM - Stem Cells and Metabolism Research Program
Faculty of Medicine
University of Helsinki
Date: 2019-11
Language: eng
Number of pages: 10
Belongs to series: Brain Research Bulletin
ISSN: 0361-9230
DOI: https://doi.org/10.1016/j.brainresbull.2019.08.008
URI: http://hdl.handle.net/10138/318290
Abstract: Sleep fragmentation (SF) commonly occurs in several pathologic conditions and is especially associated with impairments of hippocampus-dependent neurocognitive functions. Although the effects of SF on hippocampus in terms of protein or gene levels were examined in several studies, the impact of SF at the metabolite level has not been investigated. Thus, in this study, the differentially expressed large-scale metabolite profiles of hippocampus in a rat model of SF were investigated using untargeted metabolomics approaches. Forty-eight rats were divided into the following 4 groups: 4-day SF group, 4-day exercise control (EC) group, 15-day SF group, and 15-day EC group (n = 12, each). SF was accomplished by forced exercise using a walking wheel system with 30-s on/90-s off cycles, and EC condition was set at 10-min on/30-min off. The metabolite profiles of rat hippocampi in the SF and EC groups were analyzed using liquid chromatography/mass spectrometry. Multivariate analysis revealed distinctive metabolic profiles and marker signals between the SF and corresponding EC groups. Metabolic changes were significant only in the 15-day SF group. In the 15-day SF group, L-tryptophan, myristoylcarnitine, and palmitoylcarnitine were significantly increased, while adenosine monophosphate, hypoxanthine, L-glutamate, L-aspartate, L-methionine, and glycerophosphocholine were decreased compared to the EC group. The alanine, aspartate, and glutamate metabolism pathway was observed as the common key pathway in the 15-day SF groups. The results from this untargeted metabolomics study provide a perspective on metabolic impact of SF on the hippocampus.
Subject: sleep fragmentation
untargeted metabolomics
3112 Neurosciences
Peer reviewed: Yes
Rights: cc_by_nc_nd
Usage restriction: openAccess
Self-archived version: acceptedVersion

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