Oncogenic Potential of Bisphenol A and Common Environmental Contaminants in Human Mammary Epithelial Cells

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dc.contributor.author Nair, Vidhya A.
dc.contributor.author Valo, Satu
dc.contributor.author Peltomäki, Päivi
dc.contributor.author Bajbouj, Khuloud
dc.contributor.author Abdel-Rahman, Wael M.
dc.date.accessioned 2020-08-19T07:05:02Z
dc.date.available 2020-08-19T07:05:02Z
dc.date.issued 2020-05
dc.identifier.citation Nair , V A , Valo , S , Peltomäki , P , Bajbouj , K & Abdel-Rahman , W M 2020 , ' Oncogenic Potential of Bisphenol A and Common Environmental Contaminants in Human Mammary Epithelial Cells ' , International Journal of Molecular Sciences , vol. 21 , no. 10 , 3735 . https://doi.org/10.3390/ijms21103735
dc.identifier.other PURE: 142576513
dc.identifier.other PURE UUID: 52e70ee8-9500-4f6e-8b25-6d08a5362e96
dc.identifier.other WOS: 000539312100331
dc.identifier.other ORCID: /0000-0001-8819-2980/work/79183503
dc.identifier.uri http://hdl.handle.net/10138/318400
dc.description.abstract There is an ample epidemiological evidence to support the role of environmental contaminants such as bisphenol A (BPA) in breast cancer development but the molecular mechanisms of their action are still not fully understood. Therefore, we sought to analyze the effects of three common contaminants (BPA; 4-tert-octylphenol, OP; hexabromocyclododecane, HBCD) on mammary epithelial cell (HME1) and MCF7 breast cancer cell line. We also supplied some data on methoxychlor, MXC; 4-nonylphenol, NP; and 2-amino-1-methyl-6-phenylimidazo [4-b] pyridine, PhIP. We focused on testing the prolonged (two months) exposure to low nano-molar concentrations (0.0015-0.0048 nM) presumed to be oncogenic and found that they induced DNA damage (evidenced by upregulation of pH2A.X, pCHK1, pCHK2, p-P53) and disrupted the cell cycle. Some agents induced epigenetic (methylation) changes of tumor suppressor genes TIMP3, CHFR, ESR1, IGSF4, CDH13, and GSTP1. Obviously, the accumulation of these molecular alterations is an essential base for cancer development. Consistent with this, we observed that these agents increased cellular invasiveness through collagen. Cellular abilities to form colonies in soft agar were increased for MCF7. Toxic agents induced phosphorylation of protein kinase such as EGFR, CREB, STAT6, c-Jun, STAT3, HSP6, HSP27, AMPK alpha 1, FAK, p53, GSK-3 alpha/beta, and P70S6 in HME1. Most of these proteins are involved in potential oncogenic pathways. Overall, these data clarify the molecular alterations that can be induced by some common environmental contaminants in mammary epithelial cells which could be a foundation to understand environmental carcinogenesis. en
dc.format.extent 16
dc.language.iso eng
dc.relation.ispartof International Journal of Molecular Sciences
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject bisphenol A
dc.subject breast cancer
dc.subject hexabromocyclododecane
dc.subject methoxychlor
dc.subject nonylphenol
dc.subject octylphenol
dc.subject CANCER CELLS
dc.subject BREAST
dc.subject GROWTH
dc.subject COLON
dc.subject 3111 Biomedicine
dc.subject 1182 Biochemistry, cell and molecular biology
dc.title Oncogenic Potential of Bisphenol A and Common Environmental Contaminants in Human Mammary Epithelial Cells en
dc.type Article
dc.contributor.organization Department of Medical and Clinical Genetics
dc.contributor.organization University of Helsinki
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.3390/ijms21103735
dc.relation.issn 1422-0067
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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