Multiplexed High-Throughput Serological Assay for Human Enteroviruses

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Saarinen , N V V , Lehtonen , J , Veijola , R , Lempainen , J , Knip , M , Hyöty , H , Laitinen , O H & Hytönen , V P 2020 , ' Multiplexed High-Throughput Serological Assay for Human Enteroviruses ' , Microorganisms , vol. 8 , no. 6 , 963 .

Title: Multiplexed High-Throughput Serological Assay for Human Enteroviruses
Author: Saarinen, Niila V. V.; Lehtonen, Jussi; Veijola, Riitta; Lempainen, Johanna; Knip, Mikael; Hyöty, Heikki; Laitinen, Olli H.; Hytönen, Vesa P.
Contributor organization: HUS Children and Adolescents
Children's Hospital
Research Programs Unit
University of Helsinki
CAMM - Research Program for Clinical and Molecular Metabolism
Faculty of Medicine
Date: 2020-06
Language: eng
Number of pages: 16
Belongs to series: Microorganisms
ISSN: 2076-2607
Abstract: Immunological assays detecting antibodies against enteroviruses typically use a single enterovirus serotype as antigen. This limits the ability of such assays to detect antibodies against different enterovirus types and to detect possible type-specific variation in antibody responses. We set out to develop a multiplexed assay for simultaneous detection of antibodies against multiple enterovirus and rhinovirus types encompassing all human infecting species. Seven recombinant VP1 proteins from enteroviruses EV-A to EV-D and rhinoviruses RV-A to RV-C species were produced. Using Meso Scale Diagnostics U-PLEX platform we were able to study antibody reactions against these proteins as well as non-structural enterovirus proteins in a single well with 140 human serum samples. Adults had on average 33-fold stronger antibody responses to these antigens (p<10(-11)) compared to children, but children had less cross-reactivity between different enterovirus types. The results suggest that this new high-throughput assay offers clear benefits in the evaluation of humoral enterovirus immunity in children, giving more exact information than assays that are based on a single enterovirus type as antigen.
Description: Correction: Volume: 8 Issue: 9, Article Number: 1426 DOI: 10.3390/microorganisms8091426 Published: SEP 2020
Subject: enterovirus
multiplex immunoassay
11832 Microbiology and virology
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion

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