The Podovirus phi 80-18 Targets the Pathogenic American Biotype 1B Strains of Yersinia enterocolitica

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Filik , K , Szermer-Olearnik , B , Wernecki , M , Happonen , L J , Pajunen , M I , Nawaz , A , Qasim , M S , Jun , J W , Mattinen , L , Skurnik , M & Brzozowska , E 2020 , ' The Podovirus phi 80-18 Targets the Pathogenic American Biotype 1B Strains of Yersinia enterocolitica ' , Frontiers in Microbiology , vol. 11 , 1356 .

Title: The Podovirus phi 80-18 Targets the Pathogenic American Biotype 1B Strains of Yersinia enterocolitica
Author: Filik, Karolina; Szermer-Olearnik, Bozena; Wernecki, Maciej; Happonen, Lotta J.; Pajunen, Maria I.; Nawaz, Ayesha; Qasim, Muhammad Suleman; Jun, Jin Woo; Mattinen, Laura; Skurnik, Mikael; Brzozowska, Ewa
Contributor organization: Institute of Biotechnology
University of Helsinki
Glycoscience Group
HUMI - Human Microbiome Research
Research Programs Unit
Faculty of Medicine
Department of Bacteriology and Immunology
Molecular and Integrative Biosciences Research Programme
Faculty of Biological and Environmental Sciences
Helsinki One Health (HOH)
Mikael Skurnik / Principal Investigator
RNAcious laboratory
Date: 2020-06-19
Language: eng
Number of pages: 13
Belongs to series: Frontiers in Microbiology
ISSN: 1664-302X
Abstract: We report here the complete genome sequence and characterization ofYersiniabacteriophage vB_YenP_phi 80-18. phi 80-18 was isolated in 1991 using aY. enterocoliticaserotype O:8 strain 8081 as a host from a sewage sample in Turku, Finland, and based on its morphological and genomic features is classified as a podovirus. The genome is 42 kb in size and has 325 bp direct terminal repeats characteristic for podoviruses. The genome contains 57 predicted genes, all encoded in the forward strand, of which 29 showed no similarity to any known genes. Phage particle proteome analysis identified altogether 24 phage particle-associated proteins (PPAPs) including those identified as structural proteins such as major capsid, scaffolding and tail component proteins. In addition, also the DNA helicase, DNA ligase, DNA polymerase, 5 '-exonuclease, and the lytic glycosylase proteins were identified as PPAPs, suggesting that they might be injected together with the phage genome into the host cell to facilitate the take-over of the host metabolism. The phage-encoded RNA-polymerase and DNA-primase were not among the PPAPs. Promoter search predicted the presence of four phage and eleven host RNA polymerase -specific promoters in the genome, suggesting that early transcription of the phage is host RNA-polymerase dependent and that the phage RNA polymerase takes over later. The phage tolerates pH values between 2 and 12, and is stable at 50 degrees C but is inactivated at 60 degrees C. It grows slowly with a 50 min latent period and has apparently a low burst size. Electron microscopy revealed that the phage has a head diameter of about 60 nm, and a short tail of 20 nm. Whole-genome phylogenetic analysis confirmed that phi 80-18 belongs to theAutographivirinaesubfamily of thePodoviridaefamily, that it is 93.2% identical toYersiniaphage fHe-Yen3-01. Host range analysis showed that phi 80-18 can infect in addition toY. enterocoliticaserotype O:8 strains also strains of serotypes O:4, O:4,32, O:20 and O:21, the latter ones representing similar toY. enterocoliticaserotype O:8, the American pathogenic biotype 1B strains. In conclusion, the phage phi 80-18 is a promising candidate for the biocontrol of the American biotype 1BY. enterocolitica.
Subject: bacteriophage
Yersinia enterocolitica
phage biocontrol
3111 Biomedicine
11832 Microbiology and virology
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion

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