Carbon Nanomaterials Promote M1/M2 Macrophage Activation

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http://hdl.handle.net/10138/318507

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Kinaret , P A S , Scala , G , Federico , A , Sund , J & Greco , D 2020 , ' Carbon Nanomaterials Promote M1/M2 Macrophage Activation ' , Small , vol. 16 , no. 21 , 1907609 . https://doi.org/10.1002/smll.201907609

Title: Carbon Nanomaterials Promote M1/M2 Macrophage Activation
Author: Kinaret, Pia Anneli Sofia; Scala, Giovanni; Federico, Antonio; Sund, Jukka; Greco, Dario
Contributor: University of Helsinki, Institute of Biotechnology
University of Helsinki, Institute of Biotechnology
Date: 2020-05
Language: eng
Number of pages: 12
Belongs to series: Small
ISSN: 1613-6810
URI: http://hdl.handle.net/10138/318507
Abstract: Toxic effects of certain carbon nanomaterials (CNM) have been observed in several exposure scenarios both in vivo and in vitro. However, most of the data currently available has been generated in a high-dose/acute exposure setup, limiting the understanding of their immunomodulatory mechanisms. Here, macrophage-like THP-1 cells, exposed to ten different CNM for 48 h in low-cytotoxic concentration of 10 mu g mL(-1), are characterized by secretion of different cytokines and global transcriptional changes. Subsequently, the relationships between cytokine secretion and transcriptional patterns are modeled, highlighting specific pathways related to alternative macrophage activation. Finally, time- and dose-dependent activation of transcription and secretion of M1 marker genes IL-1 beta and tumor necrosis factor, and M2 marker genes IL-10 and CSF1 is confirmed among the three most responsive CNM, with concentrations of 5, 10, and 20 mu g mL(-1) at 24, 48, and 72 h of exposure. These results underline CNM effects on the formation of cell microenvironment and gene expression leading to specific patterns of macrophage polarization. Taken together, these findings imply that, instead of a high and toxic CNM dose, a sub-lethal dose in controlled exposure setup can be utilized to alter the cell microenvironment and program antigen presenting cells, with fascinating implications for novel therapeutic strategies.
Subject: EXPRESSION
GRAPHENE
HUMAN-MONOCYTES
IN-VITRO
MECHANISMS
NANOPARTICLES
NANOTUBES
RESPONSES
TOXICITY
TUMOR-NECROSIS-FACTOR
engineered nanomaterials
immunomodulation
macrophage polarization
microenvironment
toxicogenomics
220 Industrial biotechnology
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