Lignans in Knotwood of Norway Spruce : Localisation with Soft X-ray Microscopy and Scanning Transmission Electron Microscopy with Energy Dispersive X-ray Spectroscopy

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http://hdl.handle.net/10138/318557

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Mansikkala , T , Patanen , M , Karkonen , A , Korpinen , R , Pranovich , A , Ohigashi , T , Swaraj , S , Seitsonen , J , Ruokolainen , J , Huttula , M , Saranpaa , P & Piispanen , R 2020 , ' Lignans in Knotwood of Norway Spruce : Localisation with Soft X-ray Microscopy and Scanning Transmission Electron Microscopy with Energy Dispersive X-ray Spectroscopy ' , Molecules , vol. 25 , no. 13 , 2997 . https://doi.org/10.3390/molecules25132997

Title: Lignans in Knotwood of Norway Spruce : Localisation with Soft X-ray Microscopy and Scanning Transmission Electron Microscopy with Energy Dispersive X-ray Spectroscopy
Author: Mansikkala, Tuomas; Patanen, Minna; Karkonen, Anna; Korpinen, Risto; Pranovich, Andrey; Ohigashi, Takuji; Swaraj, Sufal; Seitsonen, Jani; Ruokolainen, Janne; Huttula, Marko; Saranpaa, Pekka; Piispanen, Riikka
Contributor: University of Helsinki, Department of Agricultural Sciences
Date: 2020-07
Language: eng
Number of pages: 22
Belongs to series: Molecules
ISSN: 1420-3049
URI: http://hdl.handle.net/10138/318557
Abstract: Lignans are bioactive compounds that are especially abundant in the Norway spruce (Picea abiesL. Karst.) knotwood. By combining a variety of chromatographic, spectroscopic and imaging techniques, we were able to quantify, qualify and localise the easily extractable lignans in the xylem tissue. The knotwood samples contained 15 different lignans according to the gas chromatography-mass spectrometry analysis. They comprised 16% of the knotwood dry weight and 82% of the acetone extract. The main lignans were found to be hydroxymatairesinols HMR1 and HMR2. Cryosectioned and resin-embedded ultrathin sections of the knotwood were analysed with scanning transmission X-ray microscopy (STXM). Cryosectioning was found to retain only lignan residues inside the cell lumina. In the resin-embedded samples, lignan was interpreted to be unevenly distributed inside the cell lumina, and partially confined in deposits which were either readily present in the lumina or formed when OsO(4)used in staining reacted with the lignans. Furthermore, the multi-technique characterisation enabled us to obtain information on the chemical composition of the structural components of knotwood. A simple spectral analysis of the STXM data gave consistent results with the gas chromatographic methods about the relative amounts of cell wall components (lignin and polysaccharides). The STXM analysis also indicated that a torus of a bordered pit contained aromatic compounds, possibly lignin.
Subject: Picea abies
knotwood
extractives
hydroxymatairesinol
lignin
resin-embedding
cryosectioning
STXM
STEM-EDS
osmium tetroxide
ABIES L. KARST.
CELL-WALL
WOOD
LIGNIN
HYDROXYMATAIRESINOL
HYDROGENOLYSIS
LIGNIFICATION
TOPOCHEMISTRY
TRANSITION
CHEMISTRY
4112 Forestry
11831 Plant biology
1182 Biochemistry, cell and molecular biology
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