Nuclear proteome of virus-infected and healthy potato leaves

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http://hdl.handle.net/10138/318759

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Rajamaki , M-L , Sikorskaite-Gudziuniene , S , Sarmah , N , Varjosalo , M & Valkonen , J P T 2020 , ' Nuclear proteome of virus-infected and healthy potato leaves ' , BMC Plant Biology , vol. 20 , no. 1 , 355 . https://doi.org/10.1186/s12870-020-02561-7

Julkaisun nimi: Nuclear proteome of virus-infected and healthy potato leaves
Tekijä: Rajamaki, Minna-Liisa; Sikorskaite-Gudziuniene, Sidona; Sarmah, Nandita; Varjosalo, Markku; Valkonen, Jari P. T.
Tekijän organisaatio: Department of Agricultural Sciences
Plant Pathology and Virology
Plant Production Sciences
Molecular Systems Biology
Institute of Biotechnology
Viikki Plant Science Centre (ViPS)
Päiväys: 2020-07-29
Kieli: eng
Sivumäärä: 16
Kuuluu julkaisusarjaan: BMC Plant Biology
ISSN: 1471-2229
DOI-tunniste: https://doi.org/10.1186/s12870-020-02561-7
URI: http://hdl.handle.net/10138/318759
Tiivistelmä: BackgroundInfection of plants by viruses interferes with expression and subcellular localization of plant proteins. Potyviruses comprise the largest and most economically damaging group of plant-infecting RNA viruses. In virus-infected cells, at least two potyviral proteins localize to nucleus but reasons remain partly unknown.ResultsIn this study, we examined changes in the nuclear proteome of leaf cells from a diploid potato line (Solanum tuberosum L.) after infection with potato virus A (PVA; genus Potyvirus; Potyviridae) and compared the data with that acquired for healthy leaves. Gel-free liquid chromatography-coupled to tandem mass spectrometry was used to identify 807 nuclear proteins in the potato line v2-108; of these proteins, 370 were detected in at least two samples of healthy leaves. A total of 313 proteins were common in at least two samples of healthy and PVA-infected leaves; of these proteins, 8 showed differential accumulation. Sixteen proteins were detected exclusively in the samples from PVA-infected leaves, whereas other 16 proteins were unique to healthy leaves. The protein Dnajc14 was only detected in healthy leaves, whereas different ribosomal proteins, ribosome-biogenesis proteins, and RNA splicing-related proteins were over-represented in the nuclei of PVA-infected leaves. Two virus-encoded proteins were identified in the samples of PVA-infected leaves.ConclusionsOur results show that PVA infection alters especially ribosomes and splicing-related proteins in the nucleus of potato leaves. The data increase our understanding of potyvirus infection and the role of nucleus in infection. To our knowledge, this is the first study of the nuclear proteome of potato leaves and one of the few studies of changes occurring in nuclear proteomes in response to plant virus infection.
Avainsanat: Potato virus A
Potyvirus
Potato
Solanum
Nucleus
Proteome
TURNIP-MOSAIC-VIRUS
DEPENDENT RNA-POLYMERASE
GENOME-WIDE ANALYSIS
RIBOSOMAL-PROTEINS
INCLUSION PROTEIN
TOBACCO
TRANSPORT
VPG
ACCUMULATION
NUCLEOLUS
11832 Microbiology and virology
11831 Plant biology
1182 Biochemistry, cell and molecular biology
Vertaisarvioitu: Kyllä
Tekijänoikeustiedot: cc_by
Pääsyrajoitteet: openAccess
Rinnakkaistallennettu versio: publishedVersion


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