Nuclear proteome of virus-infected and healthy potato leaves

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dc.contributor.author Rajamaki, Minna-Liisa
dc.contributor.author Sikorskaite-Gudziuniene, Sidona
dc.contributor.author Sarmah, Nandita
dc.contributor.author Varjosalo, Markku
dc.contributor.author Valkonen, Jari P. T.
dc.date.accessioned 2020-08-28T13:01:02Z
dc.date.available 2020-08-28T13:01:02Z
dc.date.issued 2020-07-29
dc.identifier.citation Rajamaki , M-L , Sikorskaite-Gudziuniene , S , Sarmah , N , Varjosalo , M & Valkonen , J P T 2020 , ' Nuclear proteome of virus-infected and healthy potato leaves ' , BMC Plant Biology , vol. 20 , no. 1 , 355 . https://doi.org/10.1186/s12870-020-02561-7
dc.identifier.other PURE: 143107841
dc.identifier.other PURE UUID: 65911e6d-e4ef-473f-a548-534d7cef848f
dc.identifier.other WOS: 000557708900003
dc.identifier.other ORCID: /0000-0002-7952-0433/work/79517604
dc.identifier.other ORCID: /0000-0002-1340-9732/work/79519168
dc.identifier.other ORCID: /0000-0002-3704-9911/work/79519609
dc.identifier.uri http://hdl.handle.net/10138/318759
dc.description.abstract BackgroundInfection of plants by viruses interferes with expression and subcellular localization of plant proteins. Potyviruses comprise the largest and most economically damaging group of plant-infecting RNA viruses. In virus-infected cells, at least two potyviral proteins localize to nucleus but reasons remain partly unknown.ResultsIn this study, we examined changes in the nuclear proteome of leaf cells from a diploid potato line (Solanum tuberosum L.) after infection with potato virus A (PVA; genus Potyvirus; Potyviridae) and compared the data with that acquired for healthy leaves. Gel-free liquid chromatography-coupled to tandem mass spectrometry was used to identify 807 nuclear proteins in the potato line v2-108; of these proteins, 370 were detected in at least two samples of healthy leaves. A total of 313 proteins were common in at least two samples of healthy and PVA-infected leaves; of these proteins, 8 showed differential accumulation. Sixteen proteins were detected exclusively in the samples from PVA-infected leaves, whereas other 16 proteins were unique to healthy leaves. The protein Dnajc14 was only detected in healthy leaves, whereas different ribosomal proteins, ribosome-biogenesis proteins, and RNA splicing-related proteins were over-represented in the nuclei of PVA-infected leaves. Two virus-encoded proteins were identified in the samples of PVA-infected leaves.ConclusionsOur results show that PVA infection alters especially ribosomes and splicing-related proteins in the nucleus of potato leaves. The data increase our understanding of potyvirus infection and the role of nucleus in infection. To our knowledge, this is the first study of the nuclear proteome of potato leaves and one of the few studies of changes occurring in nuclear proteomes in response to plant virus infection. en
dc.format.extent 16
dc.language.iso eng
dc.relation.ispartof BMC Plant Biology
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject Potato virus A
dc.subject Potyvirus
dc.subject Potato
dc.subject Solanum
dc.subject Nucleus
dc.subject Proteome
dc.subject TURNIP-MOSAIC-VIRUS
dc.subject DEPENDENT RNA-POLYMERASE
dc.subject GENOME-WIDE ANALYSIS
dc.subject RIBOSOMAL-PROTEINS
dc.subject INCLUSION PROTEIN
dc.subject TOBACCO
dc.subject TRANSPORT
dc.subject VPG
dc.subject ACCUMULATION
dc.subject NUCLEOLUS
dc.subject 11832 Microbiology and virology
dc.subject 11831 Plant biology
dc.subject 1182 Biochemistry, cell and molecular biology
dc.title Nuclear proteome of virus-infected and healthy potato leaves en
dc.type Article
dc.contributor.organization Department of Agricultural Sciences
dc.contributor.organization Plant Pathology and Virology
dc.contributor.organization Plant Production Sciences
dc.contributor.organization Molecular Systems Biology
dc.contributor.organization Institute of Biotechnology
dc.contributor.organization Viikki Plant Science Centre (ViPS)
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1186/s12870-020-02561-7
dc.relation.issn 1471-2229
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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