Chikungunya virus infections in Finnish travellers 2009-2019 : Infection Ecology & Epidemiology

Show full item record



Permalink

http://hdl.handle.net/10138/318896

Citation

Jääskeläinen , A J , Kareinen , L , Smura , T , Kallio-Kokko , H & Vapalahti , O 2020 , ' Chikungunya virus infections in Finnish travellers 2009-2019 : Infection Ecology & Epidemiology ' , Infection ecology & epidemiology , vol. 10 , no. 1 , 1798096 . https://doi.org/10.1080/20008686.2020.1798096

Title: Chikungunya virus infections in Finnish travellers 2009-2019 : Infection Ecology & Epidemiology
Author: Jääskeläinen, A. J.; Kareinen, L.; Smura, T.; Kallio-Kokko, H.; Vapalahti, O.
Contributor: University of Helsinki, Medicum
University of Helsinki, Department of Virology
University of Helsinki, HUSLAB
University of Helsinki, HUSLAB
University of Helsinki, Doctoral Programme in Microbiology and Biotechnology
Date: 2020-08-26
Language: eng
Belongs to series: Infection ecology & epidemiology
ISSN: 2000-8686
URI: http://hdl.handle.net/10138/318896
Abstract: ABSTRACT The mosquito-borne chikungunya virus (CHIKV) causes an acute febrile illness with rash, joint and muscle pain.A realtime RT-PCR assay for CHIKV detecting non-structural protein (nsP2; CHIKV nsP2-RT-qPCR) was set up. All the serodiagnosed CHIKV cases detected during 2009-2019 in Finland were screened with the assay, followed by isolations attempts and sequencing using Sanger and next generation sequencing (NGS). To validate the assay external and in-house quality control samples were used and all were correctly identified. Specificity of the assay was 100%. Assay was sensitive to detect CHIKV RNA in dilution of 10-8.During years 2009-2019 34 patients were diagnosed for acute CHIKV infection. Twelve out of 34 cases were positive by CHIKV nsP2-RT-qPCR.Two CHIKV isolations succeeded from two individuals infected originally in Thailand, 2019. From 12 CHIKV nsP2-RT-qPCR positive samples, five (42%) CHIKVs were successfully sequenced. In this study, CHIKVs from year 2019 clustered with CHIKV ECSA-lineage forming sub-cluster with strains from ones detected in Bangladesh 2017, and the ones from Jamaica (2014) within Asian lineage showing highest similarity to strains detected in Caribbean outbreak 2013-15.  Majority of the CHIKV infections detected in Finland originates from Asia and virus lineages reflect the global circulation of the pathogen.
Subject: 3111 Biomedicine
11832 Microbiology and virology
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
Chikungunya_vir ... h_travellers_2009_2019.pdf 887.1Kb PDF View/Open

This item appears in the following Collection(s)

Show full item record