Silver, titanium dioxide, and zinc oxide nanoparticles trigger miRNA/isomiR expression changes in THP-1 cells that are proportional to their health hazard potential

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Ndika , J , Seemab , U , Poon , W-L , Fortino , V , El-Nezami , H , Karisola , P & Alenius , H 2019 , ' Silver, titanium dioxide, and zinc oxide nanoparticles trigger miRNA/isomiR expression changes in THP-1 cells that are proportional to their health hazard potential ' , Nanotoxicology , vol. 13 , no. 10 , pp. 1380-1395 . https://doi.org/10.1080/17435390.2019.1661040

Title: Silver, titanium dioxide, and zinc oxide nanoparticles trigger miRNA/isomiR expression changes in THP-1 cells that are proportional to their health hazard potential
Author: Ndika, Joseph; Seemab, Umair; Poon, Wing-Lam; Fortino, Vittorio; El-Nezami, Hani; Karisola, Piia; Alenius, Harri
Contributor: University of Helsinki, HUMI - Human Microbiome Research
University of Helsinki, HUMI - Human Microbiome Research
University of Helsinki, HUMI - Human Microbiome Research
University of Helsinki, HUMI - Human Microbiome Research
Date: 2019-11-26
Language: eng
Number of pages: 16
Belongs to series: Nanotoxicology
ISSN: 1743-5390
URI: http://hdl.handle.net/10138/319305
Abstract: After over a decade of nanosafety research, it is indisputable that the vast majority of nano-sized particles induce a plethora of adverse cellular responses - the severity of which is linked to the material's physicochemical properties. Differentiated THP-1 cells were previously exposed for 6 h and 24 h to silver, titanium dioxide, and zinc oxide nanoparticles at the maximum molar concentration at which no more than 15% cellular cytotoxicity was observed. All three nanoparticles differed in extent of induction of biological pathways corresponding to immune response signaling and metal ion homeostasis. In this study, we integrated gene and miRNA expression profiles from the same cells to propose miRNA biomarkers of adverse exposure to metal-based nanoparticles. We employed RNA sequencing together with a quantitative strategy that also enables analysis of the overlooked repertoire of length and sequence miRNA variants called isomiRs. Whilst only modest changes in expression were observed within the first 6 h of exposure, the miRNA/isomiR (miR) profiles of each nanoparticle were unique. Via canonical correlation and pathway enrichment analyses, we identified a co-regulated miR-mRNA cluster, predicted to be highly relevant for cellular response to metal ion homeostasis. These miRs were annotated to be canonical or variant isoforms of hsa-miR-142-5p, -342-3p, -5100, -6087, -6894-3p, and -7704. Hsa-miR-5100 was differentially expressed in response to each nanoparticle in both the 6 h and 24 h exposures. Taken together, this co-regulated miR-mRNA cluster could represent potential biomarkers of sub-toxic metal-based nanoparticle exposure.
Subject: Metal-based nanoparticle
bio-nano reactivity
miRNA
isomiR
MIR-142-3P FUNCTIONS
TUMOR-SUPPRESSOR
RISK-ASSESSMENT
MICRORNAS
NANOMATERIALS
MECHANISM
PATHWAYS
TOOLS
3111 Biomedicine
1182 Biochemistry, cell and molecular biology
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