Saraswat , M , Joenväärä , S , Tohmola , T , Sutinen , E , Vartiainen , V , Koli , K , Myllärniemi , M & Renkonen , R 2020 , ' Label-free plasma proteomics identifies haptoglobin-related protein as candidate marker of idiopathic pulmonary fibrosis and dysregulation of complement and oxidative pathways ' , Scientific Reports , vol. 10 , no. 1 , 7787 . https://doi.org/10.1038/s41598-020-64759-x
Title: | Label-free plasma proteomics identifies haptoglobin-related protein as candidate marker of idiopathic pulmonary fibrosis and dysregulation of complement and oxidative pathways |
Author: | Saraswat, Mayank; Joenväärä, Sakari; Tohmola, Tiialotta; Sutinen, Eva; Vartiainen, Ville; Koli, Katri; Myllärniemi, Marjukka; Renkonen, Risto |
Contributor organization: | HUSLAB Transplantation Laboratory Faculty of Medicine University of Helsinki Helsinki University Hospital Area HUS Heart and Lung Center Department of Medicine Keuhkosairauksien yksikkö INDIVIDRUG - Individualized Drug Therapy Research Programs Unit Katri Koli / Principal Investigator Clinicum Department of Bacteriology and Immunology Infection Biology Research Program Risto Renkonen / Principal Investigator |
Date: | 2020-05-08 |
Language: | eng |
Number of pages: | 11 |
Belongs to series: | Scientific Reports |
ISSN: | 2045-2322 |
DOI: | https://doi.org/10.1038/s41598-020-64759-x |
URI: | http://hdl.handle.net/10138/319352 |
Abstract: | Idiopathic pulmonary fibrosis (IPF) is a lung parenchymal disease of unknown cause usually occurring in older adults. It is a chronic and progressive condition with poor prognosis and diagnosis is largely clinical. Currently, there exist few biomarkers that can predict patient outcome or response to therapies. Together with lack of markers, the need for novel markers for the detection and monitoring of IPF, is paramount. We have performed label-free plasma proteomics of thirty six individuals, 17 of which had confirmed IPF. Proteomics data was analyzed by volcano plot, hierarchical clustering, Partial-least square discriminant analysis (PLS-DA) and Ingenuity pathway analysis. Univariate and multivariate statistical analysis overlap identified haptoglobin-related protein as a possible marker of IPF when compared to control samples (Area under the curve 0.851, ROC-analysis). LXR/RXR activation and complement activation pathways were enriched in t-test significant proteins and oxidative regulators, complement proteins and protease inhibitors were enriched in PLS-DA significant proteins. Our pilot study points towards aberrations in complement activation and oxidative damage in IPF patients and provides haptoglobin-related protein as a new candidate biomarker of IPF. |
Subject: |
ABSOLUTE QUANTIFICATION
BRONCHOALVEOLAR LAVAGE IMMUNE-COMPLEXES INFLAMMATION CHOLESTEROL DISCOVERY DIAGNOSIS ROLES MICE 3111 Biomedicine |
Peer reviewed: | Yes |
Rights: | cc_by |
Usage restriction: | openAccess |
Self-archived version: | publishedVersion |
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