Label-free plasma proteomics identifies haptoglobin-related protein as candidate marker of idiopathic pulmonary fibrosis and dysregulation of complement and oxidative pathways

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Saraswat , M , Joenväärä , S , Tohmola , T , Sutinen , E , Vartiainen , V , Koli , K , Myllärniemi , M & Renkonen , R 2020 , ' Label-free plasma proteomics identifies haptoglobin-related protein as candidate marker of idiopathic pulmonary fibrosis and dysregulation of complement and oxidative pathways ' , Scientific Reports , vol. 10 , no. 1 , 7787 . https://doi.org/10.1038/s41598-020-64759-x

Title: Label-free plasma proteomics identifies haptoglobin-related protein as candidate marker of idiopathic pulmonary fibrosis and dysregulation of complement and oxidative pathways
Author: Saraswat, Mayank; Joenväärä, Sakari; Tohmola, Tiialotta; Sutinen, Eva; Vartiainen, Ville; Koli, Katri; Myllärniemi, Marjukka; Renkonen, Risto
Contributor organization: HUSLAB
Transplantation Laboratory
Faculty of Medicine
University of Helsinki
Helsinki University Hospital Area
HUS Heart and Lung Center
Department of Medicine
Keuhkosairauksien yksikkö
INDIVIDRUG - Individualized Drug Therapy
Research Programs Unit
Katri Koli / Principal Investigator
Clinicum
Department of Bacteriology and Immunology
Infection Biology Research Program
Risto Renkonen / Principal Investigator
Date: 2020-05-08
Language: eng
Number of pages: 11
Belongs to series: Scientific Reports
ISSN: 2045-2322
DOI: https://doi.org/10.1038/s41598-020-64759-x
URI: http://hdl.handle.net/10138/319352
Abstract: Idiopathic pulmonary fibrosis (IPF) is a lung parenchymal disease of unknown cause usually occurring in older adults. It is a chronic and progressive condition with poor prognosis and diagnosis is largely clinical. Currently, there exist few biomarkers that can predict patient outcome or response to therapies. Together with lack of markers, the need for novel markers for the detection and monitoring of IPF, is paramount. We have performed label-free plasma proteomics of thirty six individuals, 17 of which had confirmed IPF. Proteomics data was analyzed by volcano plot, hierarchical clustering, Partial-least square discriminant analysis (PLS-DA) and Ingenuity pathway analysis. Univariate and multivariate statistical analysis overlap identified haptoglobin-related protein as a possible marker of IPF when compared to control samples (Area under the curve 0.851, ROC-analysis). LXR/RXR activation and complement activation pathways were enriched in t-test significant proteins and oxidative regulators, complement proteins and protease inhibitors were enriched in PLS-DA significant proteins. Our pilot study points towards aberrations in complement activation and oxidative damage in IPF patients and provides haptoglobin-related protein as a new candidate biomarker of IPF.
Subject: ABSOLUTE QUANTIFICATION
BRONCHOALVEOLAR LAVAGE
IMMUNE-COMPLEXES
INFLAMMATION
CHOLESTEROL
DISCOVERY
DIAGNOSIS
ROLES
MICE
3111 Biomedicine
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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