Coupling of quinone dynamics to proton pumping in respiratory complex I

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Haapanen , O , Reidelbach , M & Sharma , V 2020 , ' Coupling of quinone dynamics to proton pumping in respiratory complex I ' , Biochimica et Biophysica Acta. Bioenergetics , vol. 1861 , no. 12 , 148287 .

Title: Coupling of quinone dynamics to proton pumping in respiratory complex I
Author: Haapanen, Outi; Reidelbach, Marco; Sharma, Vivek
Contributor organization: Materials Physics
Department of Physics
Helsinki Institute of Life Science HiLIFE, Joint Activities
Date: 2020-12-01
Language: eng
Number of pages: 6
Belongs to series: Biochimica et Biophysica Acta. Bioenergetics
ISSN: 0005-2728
Abstract: Respiratory complex I (NADH:quinone oxidoreductase) plays a central role in generating the proton electrochemical gradient in mitochondrial and bacterial membranes, which is needed to generate ATP. Several high-resolution structures of complex I have been determined, revealing its intricate architecture and complementing the biochemical and biophysical studies. However, the molecular mechanism of long-range coupling between ubiquinone (Q) reduction and proton pumping is not known. Computer simulations have been applied to decipher the dynamics of Q molecule in the similar to 30 angstrom long Q tunnel. In this short report, we discuss the binding and dynamics of Q at computationally predicted Q binding sites, many of which are supported by structural data on complex I. We suggest that the binding of Q at these sites is coupled to proton pumping by means of conformational rearrangements in the conserved loops of core subunits.
Subject: Cell respiration
Molecular dynamics
Electron-proton coupling
114 Physical sciences
1182 Biochemistry, cell and molecular biology
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion
Funder: Valtion perusrahoitus/hankkeet
Grant number:

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