Dissecting the interaction of photosynthetic electron transfer with mitochondrial signalling and hypoxic response in the Arabidopsis rcd1 mutant

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Shapiguzov , A , Nikkanen , L , Fitzpatrick , D , Vainonen , J P , Gossens , R , Alseekh , S , Aarabi , F , Tiwari , A , Blokhina , O , Panzarova , K , Benedikty , Z , Tyystjärvi , E , Fernie , A R , Trtilek , M , Aro , E-M , Rintamäki , E & Kangasjarvi , J 2020 , ' Dissecting the interaction of photosynthetic electron transfer with mitochondrial signalling and hypoxic response in the Arabidopsis rcd1 mutant ' , Philosophical Transactions of the Royal Society. Biological Sciences , vol. 375 , no. 1801 , 20190413 . https://doi.org/10.1098/rstb.2019.0413

Title: Dissecting the interaction of photosynthetic electron transfer with mitochondrial signalling and hypoxic response in the Arabidopsis rcd1 mutant
Author: Shapiguzov, Alexey; Nikkanen, Lauri; Fitzpatrick, Duncan; Vainonen, Julia P.; Gossens, Richard; Alseekh, Saleh; Aarabi, Fayezeh; Tiwari, Arjun; Blokhina, Olga; Panzarova, Klara; Benedikty, Zuzana; Tyystjärvi, Esa; Fernie, Alisdair R.; Trtilek, Martin; Aro, Eva-Mari; Rintamäki, Eevi; Kangasjarvi, Jaakko
Contributor organization: Organismal and Evolutionary Biology Research Programme
Plant ROS-Signalling
Plant Biology
Faculty of Biological and Environmental Sciences
Biosciences
Staff Services
Viikki Plant Science Centre (ViPS)
Date: 2020-06-22
Language: eng
Number of pages: 10
Belongs to series: Philosophical Transactions of the Royal Society. Biological Sciences
ISSN: 0962-8436
DOI: https://doi.org/10.1098/rstb.2019.0413
URI: http://hdl.handle.net/10138/320200
Abstract: The Arabidopsis mutant rcd1 is tolerant to methyl viologen (MV). MV enhances the Mehler reaction, i.e. electron transfer from Photosystem I (PSI) to O-2, generating reactive oxygen species (ROS) in the chloroplast. To study the MV tolerance of rcd1, we first addressed chloroplast thiol redox enzymes potentially implicated in ROS scavenging. NADPH-thioredoxin oxidoreductase type C (NTRC) was more reduced in rcd1. NTRC contributed to the photosynthetic and metabolic phenotypes of rcd1, but did not determine its MV tolerance. We next tested rcd1 for alterations in the Mehler reaction. In rcd1, but not in the wild type, the PSI-to-MV electron transfer was abolished by hypoxic atmosphere. A characteristic feature of rcd1 is constitutive expression of mitochondrial dysfunction stimulon (MDS) genes that affect mitochondrial respiration. Similarly to rcd1, in other MDS-overexpressing plants hypoxia also inhibited the PSI-to-MV electron transfer. One possible explanation is that the MDS gene products may affect the Mehler reaction by altering the availability of O-2. In green tissues, this putative effect is masked by photosynthetic O-2 evolution. However, O-2 evolution was rapidly suppressed in MV-treated plants. Transcriptomic meta-analysis indicated that MDS gene expression is linked to hypoxic response not only under MV, but also in standard growth conditions. This article is part of the theme issue 'Retrograde signalling from endosymbiotic organelles'.
Subject: Arabidopsis thaliana
photosynthetic electron transfer
reactive oxygen species
hypoxia
mitochondrial dysfunction stimulon
retrograde signalling
CHLOROPLAST THIOREDOXIN SYSTEMS
NAC TRANSCRIPTION FACTOR
WATER-WATER CYCLE
ALTERNATIVE OXIDASE
PHOTOSYSTEM-I
OXIDATIVE STRESS
REDUCTASE C
OXYGEN
REDOX
PATHWAY
1182 Biochemistry, cell and molecular biology
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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