Hybrid red blood cell membrane coated porous silicon nanoparticles functionalized with cancer antigen induce depletion of T cells

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Rahikkala , A , Fontana , F , Bauleth-Ramos , T , Rebelo Correia , A M , Kemell , M , Seitsonen , J , Mäkilä , E , Sarmento , B , Salonen , J , Ruokolainen , J , Hirvonen , J & Santos , H A 2020 , ' Hybrid red blood cell membrane coated porous silicon nanoparticles functionalized with cancer antigen induce depletion of T cells ' , RSC Advances , vol. 10 , no. 58 , pp. 35198-35205 . https://doi.org/10.1039/D0RA05900E

Title: Hybrid red blood cell membrane coated porous silicon nanoparticles functionalized with cancer antigen induce depletion of T cells
Author: Rahikkala, Antti; Fontana, Flavia; Bauleth-Ramos, Tomás; Rebelo Correia, Alexandra Maria; Kemell, Marianna; Seitsonen, Jani; Mäkilä, Ermei; Sarmento, Bruno; Salonen, Jarno; Ruokolainen, Janne; Hirvonen, Jouni; Santos, Hélder A.
Contributor: University of Helsinki, Division of Pharmaceutical Chemistry and Technology
University of Helsinki, Nanomedicines and Biomedical Engineering
University of Helsinki, Nanomedicines and Biomedical Engineering
University of Helsinki, Department of Chemistry
University of Helsinki, Drug Research Program
University of Helsinki, Helsinki One Health (HOH)
Date: 2020-09-27
Language: eng
Number of pages: 8
Belongs to series: RSC Advances
ISSN: 2046-2069
URI: http://hdl.handle.net/10138/320337
Abstract: Erythrocyte-based drug delivery systems have been investigated for their biocompatibility, long circulation time, and capability to transport cargo all around the body, thus presenting enormous potential in medical applications. In this study, we investigated hybrid nanoparticles consisting of nano-sized autologous or allogeneic red blood cell (RBC) membranes encapsulating porous silicon nanoparticles (PSi NPs). These NPs were functionalized with a model cancer antigen TRP2, which was either expressed on the surface of the RBCs by a cell membrane-mimicking block copolymer polydimethylsiloxane-b-poly-2-methyl-2-oxazoline, or attached on the PSi NPs, thus hidden within the encapsulation. When in the presence of peripheral blood immune cells, these NPs resulted in apoptotic cell death of T cells, where the NPs having TRP2 within the encapsulation led to a stronger T cell deletion. The deletion of the T cells did not change the relative proportion of CD4+ and cytotoxic CD8+ T cells. Overall, this work shows the combination of nano-sized RBCs, PSi, and antigenic peptides may have use in the treatment of autoimmune diseases.
Subject: 116 Chemical sciences
317 Pharmacy
DENDRITIC CELLS
SURFACE-CHEMISTRY
IMMUNE-RESPONSES
IN-VITRO
DRUG-DELIVERY
RBC MEMBRANES
TRP-2
TOLERANCE
PEPTIDE
318 Medical biotechnology
DENDRITIC CELLS
SURFACE-CHEMISTRY
IMMUNE-RESPONSES
IN-VITRO
DRUG-DELIVERY
RBC MEMBRANES
TRP-2
TOLERANCE
PEPTIDE
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