Utilization of Green Formulation Technique and Efficacy Estimation on Cell Line Studies For Dual Anticancer Drug Therapy With Niosomes

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Khan , D H , Bashir , S , Correia , A , Khan , M I , Figueiredo , P , Santos , H A & Peltonen , L 2019 , ' Utilization of Green Formulation Technique and Efficacy Estimation on Cell Line Studies For Dual Anticancer Drug Therapy With Niosomes ' , International Journal of Pharmaceutics , vol. 572 , 118764 . https://doi.org/10.1016/j.ijpharm.2019.118764

Title: Utilization of Green Formulation Technique and Efficacy Estimation on Cell Line Studies For Dual Anticancer Drug Therapy With Niosomes
Author: Khan, Daulat Haleem; Bashir, Sajid; Correia, Alexandra; Khan, Muhammad Imran; Figueiredo, Patricia; Santos, Hélder A.; Peltonen, Leena
Contributor organization: Nanomedicines and Biomedical Engineering
Division of Pharmaceutical Chemistry and Technology
Drug Research Program
Divisions of Faculty of Pharmacy
Helsinki One Health (HOH)
Helsinki Institute of Life Science HiLIFE
Doctoral Programme in Drug Research
University Management
Date: 2019-12-15
Language: eng
Number of pages: 11
Belongs to series: International Journal of Pharmaceutics
ISSN: 0378-5173
DOI: https://doi.org/10.1016/j.ijpharm.2019.118764
URI: http://hdl.handle.net/10138/320373
Abstract: The aim of the present study was to prepare niosome formulations for the simultaneous encapsulation, dual drug therapy, of two anticancer drugs by the ecological probe sonication method. Poloxamer and sorbitan monostearate were used as surface active agents in niosomes, and the water soluble doxorubicin and poorly-water soluble paclitaxel were used as anticancer drugs. Thorough physicochemical analysis were performed for the niosomes, and their cytotoxicity and activity were evaluated on MCF-7 and PC3-MM2 cancer cell lines. Prepared niosomes were small in size with sizes ranging from 137 nm to 893 nm, and entrapment efficiencies were high, ranging from 91.24% to 99.99%. During the four weeks stability testing, the particle size remained stable. The niosomal formulations showed in vitro sustained drug release profiles for doxorubicin and clearly increased the dissolution rate of poorly water soluble paclitaxel. The incorporation of both the drugs into niosomes improved cell penetration and antiproliferative activity of the drugs PC3-MM2 cell lines. As a conclusion, doxorubicin and paclitaxel loaded niosome formulations resulted in relatively stable, small sized niosomes with improved drug release profiles, low toxicity, better cell penetration and antiproliferative activity. The niosomes showed synergistic effect due to the presence of both drugs, which can overcome multidrug resistance.
Subject: Antiproliferative activity
CANCER-THERAPY
CARRIERS
Cell penetration
DELIVERY SYSTEMS
DOXORUBICIN
Doxorubicin
Dual drug therapy
IN-VITRO CHARACTERIZATION
NANO-NIOSOMES
Niosomes
OPTIMIZATION
PACLITAXEL
POLYMER HYBRID NANOPARTICLES
Paclitaxel
RELEASE
317 Pharmacy
Peer reviewed: Yes
Rights: cc_by_nc_nd
Usage restriction: openAccess
Self-archived version: acceptedVersion


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