A CX3CRI Reporter hESC Line Facilitates Integrative Analysis of In-Vitro-Derived Microglia and Improved Microglia Identity upon Neuron-Glia Co-culture

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dc.contributor University of Helsinki, Neuroscience Center en
dc.contributor.author Grubman, Alexandra
dc.contributor.author Vandekolk, Teresa H.
dc.contributor.author Schröder, Jan
dc.contributor.author Sun, Guizhi
dc.contributor.author Hatwell-Humble, Jessica
dc.contributor.author Chan, Jonathan
dc.contributor.author Oksanen, Minna
dc.contributor.author Lehtonen, Sarka
dc.contributor.author Hunt, Cameron
dc.contributor.author Koistinaho, Jan E.
dc.contributor.author Nilsson, Susan K.
dc.contributor.author Haynes, John M.
dc.contributor.author Pouton, Colin W.
dc.contributor.author Polo, Jose M.
dc.date.accessioned 2020-10-16T09:25:03Z
dc.date.available 2020-10-16T09:25:03Z
dc.date.issued 2020-06-09
dc.identifier.citation Grubman , A , Vandekolk , T H , Schröder , J , Sun , G , Hatwell-Humble , J , Chan , J , Oksanen , M , Lehtonen , S , Hunt , C , Koistinaho , J E , Nilsson , S K , Haynes , J M , Pouton , C W & Polo , J M 2020 , ' A CX3CRI Reporter hESC Line Facilitates Integrative Analysis of In-Vitro-Derived Microglia and Improved Microglia Identity upon Neuron-Glia Co-culture ' , Stem cell reports , vol. 14 , no. 6 , pp. 1018-1032 . https://doi.org/10.1016/j.stemcr.2020.04.007 en
dc.identifier.issn 2213-6711
dc.identifier.other PURE: 149243939
dc.identifier.other PURE UUID: a2517c85-a771-441d-9896-ba2d132f6446
dc.identifier.other WOS: 000540604900004
dc.identifier.uri http://hdl.handle.net/10138/320383
dc.description.abstract Multiple protocols have been published for generation of iMGLs from hESCs/iPSCs. To date, there are no guides to assist researchers to determine the most appropriate methodology for microglial studies. To establish a framework to facilitate future microglial studies, we first performed a comparative transcriptional analysis between iMGLs derived using three published datasets, which allowed us to establish the baseline protocol that is most representative of bona fide human microglia. Secondly, using CRISPR to tag the classic microglial marker CX3CR1 with nanoluciferase and tdTomato, we generated and functionally validated a reporter ESC line. Finally, using this cell line, we demonstrated that co-culture of iMGL precursors with human glia and neurons enhanced transcriptional resemblance of iMGLs to ex vivo microglia. Together, our comprehensive molecular analysis and reporter cell line are a useful resource for neurobiologists seeking to use iMGLs for disease modeling and drug screening studies. en
dc.format.extent 15
dc.language.iso eng
dc.relation.ispartof Stem cell reports
dc.rights en
dc.subject PLURIPOTENT STEM-CELLS en
dc.subject DIFFERENTIATION en
dc.subject MODEL en
dc.subject ENVIRONMENT en
dc.subject 1182 Biochemistry, cell and molecular biology en
dc.title A CX3CRI Reporter hESC Line Facilitates Integrative Analysis of In-Vitro-Derived Microglia and Improved Microglia Identity upon Neuron-Glia Co-culture en
dc.type Article
dc.description.version Peer reviewed
dc.identifier.doi https://doi.org/10.1016/j.stemcr.2020.04.007
dc.type.uri info:eu-repo/semantics/other
dc.type.uri info:eu-repo/semantics/publishedVersion
dc.contributor.pbl

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