Colonic Mucosal Microbiota and Association of Bacterial Taxa with the Expression of Host Antimicrobial Peptides in Pediatric Ulcerative Colitis

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Jalanka , J , Cheng , J , Hiippala , K , Ritari , J , Salojärvi , J , Ruuska , T , Kalliomaki , M & Satokari , R 2020 , ' Colonic Mucosal Microbiota and Association of Bacterial Taxa with the Expression of Host Antimicrobial Peptides in Pediatric Ulcerative Colitis ' , International Journal of Molecular Sciences , vol. 21 , no. 17 , 6044 . https://doi.org/10.3390/ijms21176044

Title: Colonic Mucosal Microbiota and Association of Bacterial Taxa with the Expression of Host Antimicrobial Peptides in Pediatric Ulcerative Colitis
Author: Jalanka, Jonna; Cheng, Jing; Hiippala, Kaisa; Ritari, Jarmo; Salojärvi, Jarkko; Ruuska, Tarja; Kalliomaki, Marko; Satokari, Reetta
Contributor organization: HUMI - Human Microbiome Research
Faculty of Medicine
University of Helsinki
Research Programs Unit
Reetta Maria Satokari / Principal Investigator
Date: 2020-09
Language: eng
Number of pages: 13
Belongs to series: International Journal of Molecular Sciences
ISSN: 1422-0067
DOI: https://doi.org/10.3390/ijms21176044
URI: http://hdl.handle.net/10138/320384
Abstract: Inflammatory bowel diseases (IBD), ulcerative colitis (UC) and Crohn's disease (CD), are chronic debilitating disorders of unknown etiology. Over 200 genetic risk loci are associated with IBD, highlighting a key role for immunological and epithelial barrier functions. Environmental factors account for the growing incidence of IBD, and microbiota are considered as an important contributor. Microbiota dysbiosis can lead to a loss of tolerogenic immune effects and initiate or exacerbate inflammation. We aimed to study colonic mucosal microbiota and the expression of selected host genes in pediatric UC. We used high-throughput 16S rDNA sequencing to profile microbiota in colonic biopsies of pediatric UC patients (n= 26) and non-IBD controls (n= 27). The expression of 13 genes, including five for antimicrobial peptides, in parallel biopsies was assessed with qRT-PCR. The composition of microbiota between UC and non-IBD differed significantly (PCoA,p= 0.001). UC children had a decrease in Bacteroidetes and an increase in several family-level taxa including Peptostreptococcaceae and Enterobacteriaceae, which correlated negatively with the expression of antimicrobial peptides REG3G and DEFB1, respectively. Enterobacteriaceae correlated positively with the expression siderophore binding protein LCN2 and Betaproteobacteria negatively with DEFB4A expression. The results indicate that reciprocal interaction of epithelial microbiota and defense mechanisms play a role in UC.
Subject: inflammatory bowel disease
ulcerative colitis
microbiota
gene expression
host-microbe cross-talk
INFLAMMATORY BOWEL DISEASES
FECAL MICROBIOTA
INTESTINAL-MUCOSA
GUT
REVEALS
GENOME
IBD
1182 Biochemistry, cell and molecular biology
116 Chemical sciences
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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