A novel negevirus isolated from Aedes vexans mosquitoes in Finland

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Suvanto , M T , Truong Nguyen , P , Uusitalo , R , Korhonen , E M , Faolotto , G , Vapalahti , O , Huhtamo , E & Smura , T 2020 , ' A novel negevirus isolated from Aedes vexans mosquitoes in Finland ' , Archives of Virology , vol. 165 , no. 12 , pp. 2989–2992 . https://doi.org/10.1007/s00705-020-04810-4

Title: A novel negevirus isolated from Aedes vexans mosquitoes in Finland
Author: Suvanto, Maija T.; Truong Nguyen, Phuoc; Uusitalo, Ruut; Korhonen, Essi M.; Faolotto, Giulia; Vapalahti, Olli; Huhtamo, Eili; Smura, Teemu
Contributor: University of Helsinki, Department of Virology
University of Helsinki, Department of Geosciences and Geography
University of Helsinki, Viral Zoonosis Research Unit
University of Helsinki, Helsinki One Health (HOH)
University of Helsinki, Department of Virology
University of Helsinki, Department of Virology
Date: 2020-12
Language: eng
Number of pages: 4
Belongs to series: Archives of Virology
ISSN: 0304-8608
URI: http://hdl.handle.net/10138/320668
Abstract: Negeviruses are insect-specific enveloped RNA viruses that have been detected in mosquitoes and sandflies from various geographical locations. Here, we describe a new negevirus from Northern Europe, isolated from pool ofAedes vexansmosquitoes collected in Finland, designated as Mekrijarvi negevirus (MEJNV). MEJNV had a typical negevirus genome organization, is 9,740 nucleotides in length, and has a GC content of 47.53%. The MEJNV genome contains three ORFs, each containing the following identified conserved domains: ORF1 (7,068 nt) encodes a viral methyltransferase, an FtsJ-like methyltransferase, a viral RNA helicase, and an RNA-dependent RNA polymerase, ORF2 (1,242 nt) encodes a putative virion glycoprotein, and ORF3 (660 nt) encodes a putative virion membrane protein. A distinctive feature relative to other currently known negeviruses is a 7-nucleotide-long overlap between ORF1 and ORF2. MEJNV shares the highest sequence identity with Ying Kou virus from China, with 67.71% nucleotide and 75.19% and 59.00% amino acid sequence identity in ORF 1 and ORF 2, respectively. ORF3 had the highest amino acid sequence similarity to Daeseongdong virus 1 and negevirus Nona 1, both with 77.61% identity, and to Ying Kou virus, with 71.22% identity. MEJNV is currently the northernmost negevirus described. Our report supports the view that negeviruses are a globally distributed, diverse group of viruses that can be found from mosquitoes in a wide range of terrestrial biomes from tropical to boreal forests.
Subject: INSECT-SPECIFIC VIRUSES
GENETIC-CHARACTERIZATION
TAXON
11832 Microbiology and virology
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