BetaHPV E6 and E7 colocalize with NuMa in dividing keratinocytes

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dc.contributor.author Oswald, Evelyn
dc.contributor.author Kirschberg, Matthias
dc.contributor.author Aubin, Francois
dc.contributor.author Alonso, Angel
dc.contributor.author Hufbauer, Martin
dc.contributor.author Akguel, Baki
dc.contributor.author Auvinen, Eeva
dc.date.accessioned 2020-10-31T03:28:07Z
dc.date.available 2021-12-18T03:46:01Z
dc.date.issued 2019-10
dc.identifier.citation Oswald , E , Kirschberg , M , Aubin , F , Alonso , A , Hufbauer , M , Akguel , B & Auvinen , E 2019 , ' BetaHPV E6 and E7 colocalize with NuMa in dividing keratinocytes ' , Virus Genes , vol. 55 , no. 5 , pp. 600-609 . https://doi.org/10.1007/s11262-019-01685-9
dc.identifier.other PURE: 127742000
dc.identifier.other PURE UUID: cfe67dc7-e8f0-49f3-96a9-ec8b79e590be
dc.identifier.other WOS: 000486222300004
dc.identifier.other ORCID: /0000-0002-2094-985X/work/63999395
dc.identifier.uri http://hdl.handle.net/10138/320952
dc.description.abstract Human papillomaviruses (HPVs) of genus betapapillomavirus (betaHPV) are implicated in skin carcinogenesis, but their exact role in keratinocyte transformation is poorly understood. We show an interaction of HPV5 and HPV8 oncoproteins E6 and E7 with the nuclear mitotic apparatus protein 1 (NuMA). Binding of E6 or E7 to NuMA induces little aneuploidy, cell cycle alterations, or aberrant centrosomes. Intracellular localization of NuMA is not altered by E6 and E7 expression in 2D cultures. However, the localization profile is predominantly cytoplasmic in 3D organotypic skin models. Both viral proteins colocalize with NuMA in interphase cells, while only E7 colocalizes with NuMA in mitotic cells. Intriguingly, a small subset of cells shows E7 at only one spindle pole, whereas NuMA is present at both poles. This dissimilar distribution of E7 at the spindle poles may alter cell differentiation, which may in turn be relevant for betaHPV-induced skin carcinogenesis. en
dc.format.extent 10
dc.language.iso eng
dc.relation.ispartof Virus Genes
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject Human papillomavirus
dc.subject Betapapillomavirus
dc.subject Nuclear mitotic apparatus protein 1
dc.subject HUMAN-PAPILLOMAVIRUS TYPE-16
dc.subject EARLY GENES
dc.subject PROTEIN
dc.subject EXPRESSION
dc.subject DIFFERENTIATION
dc.subject CHROMOSOME
dc.subject ORGANIZATION
dc.subject CENTROSOME
dc.subject ABROGATE
dc.subject INVASION
dc.subject 3111 Biomedicine
dc.title BetaHPV E6 and E7 colocalize with NuMa in dividing keratinocytes en
dc.type Article
dc.contributor.organization University of Helsinki
dc.contributor.organization Eeva Auvinen / Principal Investigator
dc.contributor.organization University Management
dc.contributor.organization Medicum
dc.contributor.organization HUSLAB
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1007/s11262-019-01685-9
dc.relation.issn 0920-8569
dc.rights.accesslevel openAccess
dc.type.version publishedVersion
dc.identifier.url https://rdcu.be/bVDWc

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