Cis-regulatory differences in isoform expression associate with life history strategy variation in Atlantic salmon

Visa fullständig post



Permalänk

http://hdl.handle.net/10138/321074

Citation

Verta , J-P , Debes , P , Piavchenko , N , Ruokolainen , A , Ovaskainen , O S , Moustakas-Verho , J , Tillanen , S I , Parre , N , Aykanat , T , Erkinaro , J & Primmer , C 2020 , ' Cis-regulatory differences in isoform expression associate with life history strategy variation in Atlantic salmon ' , PLoS Genetics , vol. 16 , no. 9 , 1009055 . https://doi.org/10.1371/journal.pgen.1009055

Titel: Cis-regulatory differences in isoform expression associate with life history strategy variation in Atlantic salmon
Författare: Verta, Jukka-Pekka; Debes, Paul; Piavchenko, Nikolai; Ruokolainen, Annukka; Ovaskainen, Outi Sinikka; Moustakas-Verho, Jacqueline; Tillanen, Seija Iiris; Parre, Noora; Aykanat, Tutku; Erkinaro, Jaakko; Primmer, Craig
Upphovmannens organisation: Evolution, Conservation, and Genomics
Organismal and Evolutionary Biology Research Programme
Research Services
Genetics
Helsinki Institute of Sustainability Science (HELSUS)
Institute of Biotechnology
Datum: 2020-09
Språk: eng
Sidantal: 23
Tillhör serie: PLoS Genetics
ISSN: 1553-7390
DOI: https://doi.org/10.1371/journal.pgen.1009055
Permanenta länken (URI): http://hdl.handle.net/10138/321074
Abstrakt: A major goal in biology is to understand how evolution shapes variation in individual life histories. Genome-wide association studies have been successful in uncovering genome regions linked with traits underlying life history variation in a range of species. However, lack of functional studies of the discovered genotype-phenotype associations severely restrains our understanding how alternative life history traits evolved and are mediated at the molecular level. Here, we report acis-regulatory mechanism whereby expression of alternative isoforms of the transcription co-factorvestigial-like 3(vgll3) associate with variation in a key life history trait, age at maturity, in Atlantic salmon (Salmo salar). Using a common-garden experiment, we first show thatvgll3genotype associates with puberty timing in one-year-old salmon males. By way of temporal sampling ofvgll3expression in ten tissues across the first year of salmon development, we identify a pubertal transition invgll3expression where maturation coincided with a 66% reduction in testicularvgll3expression. Thelatematuration allele was not only associated with a tendency to delay puberty, but also with expression of a rare transcript isoform ofvgll3pre-puberty. By comparing absolutevgll3mRNA copies in heterozygotes we show that the expression difference between theearlyandlatematurity alleles is largelycis-regulatory. We propose a model whereby expression of a rare isoform from thelateallele shifts the liability of its carriers towards delaying puberty. These results exemplify the potential importance of regulatory differences as a mechanism for the evolution of life history traits. Author summary Alternative life history strategies are an important source of diversity within populations and promote the maintenance of adaptive capacity and population resilience. However, in many cases the molecular basis of different life history strategies remains elusive. Age at maturity is a key adaptive life history trait in Atlantic salmon and has a relatively simple genetic basis. Using salmon age at maturity as a model, we report a mechanism whereby different transcript isoforms of the key age at maturity gene,vestigial-like 3(vgll3), associate with variation in the timing of male puberty. Our results show how gene regulatory differences in conjunction with variation in gene transcript structure can encode for complex alternative life histories.
Subject: 1184 Genetics, developmental biology, physiology
SEX-DEPENDENT DOMINANCE
ADAPTIVE EVOLUTION
VESTIGIAL-LIKE
TRADE-OFFS
GENE
DIFFERENTIATION
POPULATIONS
MODELS
LOCI
HERITABILITY
Referentgranskad: Ja
Licens: cc_by
Användningsbegränsning: openAccess
Parallelpublicerad version: publishedVersion


Filer under denna titel

Totalt antal nerladdningar: Laddar...

Filer Storlek Format Granska
journal.pgen.1009055.pdf 24.80Mb PDF Granska/Öppna

Detta dokument registreras i samling:

Visa fullständig post