Cis-regulatory differences in isoform expression associate with life history strategy variation in Atlantic salmon

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dc.contributor.author Verta, Jukka-Pekka
dc.contributor.author Debes, Paul
dc.contributor.author Piavchenko, Nikolai
dc.contributor.author Ruokolainen, Annukka
dc.contributor.author Ovaskainen, Outi Sinikka
dc.contributor.author Moustakas-Verho, Jacqueline
dc.contributor.author Tillanen, Seija Iiris
dc.contributor.author Parre, Noora
dc.contributor.author Aykanat, Tutku
dc.contributor.author Erkinaro, Jaakko
dc.contributor.author Primmer, Craig
dc.date.accessioned 2020-11-03T14:41:02Z
dc.date.available 2020-11-03T14:41:02Z
dc.date.issued 2020-09
dc.identifier.citation Verta , J-P , Debes , P , Piavchenko , N , Ruokolainen , A , Ovaskainen , O S , Moustakas-Verho , J , Tillanen , S I , Parre , N , Aykanat , T , Erkinaro , J & Primmer , C 2020 , ' Cis-regulatory differences in isoform expression associate with life history strategy variation in Atlantic salmon ' , PLoS Genetics , vol. 16 , no. 9 , 1009055 . https://doi.org/10.1371/journal.pgen.1009055
dc.identifier.other PURE: 145498271
dc.identifier.other PURE UUID: 34a4d846-43a9-406e-b519-adb5d30f1d3e
dc.identifier.other WOS: 000577085600001
dc.identifier.other ORCID: /0000-0001-9702-0945/work/83051677
dc.identifier.other ORCID: /0000-0003-1701-6124/work/83054348
dc.identifier.other ORCID: /0000-0002-3687-8435/work/83054440
dc.identifier.uri http://hdl.handle.net/10138/321074
dc.description.abstract A major goal in biology is to understand how evolution shapes variation in individual life histories. Genome-wide association studies have been successful in uncovering genome regions linked with traits underlying life history variation in a range of species. However, lack of functional studies of the discovered genotype-phenotype associations severely restrains our understanding how alternative life history traits evolved and are mediated at the molecular level. Here, we report acis-regulatory mechanism whereby expression of alternative isoforms of the transcription co-factorvestigial-like 3(vgll3) associate with variation in a key life history trait, age at maturity, in Atlantic salmon (Salmo salar). Using a common-garden experiment, we first show thatvgll3genotype associates with puberty timing in one-year-old salmon males. By way of temporal sampling ofvgll3expression in ten tissues across the first year of salmon development, we identify a pubertal transition invgll3expression where maturation coincided with a 66% reduction in testicularvgll3expression. Thelatematuration allele was not only associated with a tendency to delay puberty, but also with expression of a rare transcript isoform ofvgll3pre-puberty. By comparing absolutevgll3mRNA copies in heterozygotes we show that the expression difference between theearlyandlatematurity alleles is largelycis-regulatory. We propose a model whereby expression of a rare isoform from thelateallele shifts the liability of its carriers towards delaying puberty. These results exemplify the potential importance of regulatory differences as a mechanism for the evolution of life history traits. Author summary Alternative life history strategies are an important source of diversity within populations and promote the maintenance of adaptive capacity and population resilience. However, in many cases the molecular basis of different life history strategies remains elusive. Age at maturity is a key adaptive life history trait in Atlantic salmon and has a relatively simple genetic basis. Using salmon age at maturity as a model, we report a mechanism whereby different transcript isoforms of the key age at maturity gene,vestigial-like 3(vgll3), associate with variation in the timing of male puberty. Our results show how gene regulatory differences in conjunction with variation in gene transcript structure can encode for complex alternative life histories. en
dc.format.extent 23
dc.language.iso eng
dc.relation.ispartof PLoS Genetics
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject 1184 Genetics, developmental biology, physiology
dc.subject SEX-DEPENDENT DOMINANCE
dc.subject ADAPTIVE EVOLUTION
dc.subject VESTIGIAL-LIKE
dc.subject TRADE-OFFS
dc.subject GENE
dc.subject DIFFERENTIATION
dc.subject POPULATIONS
dc.subject MODELS
dc.subject LOCI
dc.subject HERITABILITY
dc.title Cis-regulatory differences in isoform expression associate with life history strategy variation in Atlantic salmon en
dc.type Article
dc.contributor.organization Evolution, Conservation, and Genomics
dc.contributor.organization Organismal and Evolutionary Biology Research Programme
dc.contributor.organization Research Services
dc.contributor.organization Genetics
dc.contributor.organization Helsinki Institute of Sustainability Science (HELSUS)
dc.contributor.organization Institute of Biotechnology
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1371/journal.pgen.1009055
dc.relation.issn 1553-7390
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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