Adipose-Derived Mesenchymal Stem Cells do not Affect the Invasion and Migration Potential of Oral Squamous Carcinoma Cells

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Sinha , S , Narjus-Sterba , M , Tuomainen , K , Kaur , S , Seppänen-Kaijansinkko , R , Salo , T , Mannerström , B & Al-Samadi , A 2020 , ' Adipose-Derived Mesenchymal Stem Cells do not Affect the Invasion and Migration Potential of Oral Squamous Carcinoma Cells ' , International Journal of Molecular Sciences , vol. 21 , no. 18 , 6455 . https://doi.org/10.3390/ijms21186455

Title: Adipose-Derived Mesenchymal Stem Cells do not Affect the Invasion and Migration Potential of Oral Squamous Carcinoma Cells
Author: Sinha, Snehadri; Narjus-Sterba, Matilda; Tuomainen, Katja; Kaur, Sippy; Seppänen-Kaijansinkko, Riitta; Salo, Tuula; Mannerström, Bettina; Al-Samadi, Ahmed
Contributor: University of Helsinki, Department of Oral and Maxillofacial Diseases
University of Helsinki, Department of Oral and Maxillofacial Diseases
University of Helsinki, HUS Head and Neck Center
University of Helsinki, Clinicum
University of Helsinki, HUS Head and Neck Center
University of Helsinki, HUSLAB
University of Helsinki, Medicum
University of Helsinki, Department of Oral and Maxillofacial Diseases
Date: 2020-09
Language: eng
Number of pages: 15
Belongs to series: International Journal of Molecular Sciences
ISSN: 1422-0067
URI: http://hdl.handle.net/10138/321160
Abstract: Mesenchymal stem cells (MSCs) are commonly isolated from bone marrow and adipose tissue. Depending on the tissue of origin, MSCs have different characteristics and physiological effects. In various cancer studies, MSCs have been found to have either tumor-promoting or tumor-inhibiting action. This study investigated the effect of adipose tissue-MSCs (AT-MSCs) and bone marrow-MSCs (BM-MSCs) on global long interspersed nuclear element-1 (LINE-1) methylation, the expression level of microenvironment remodeling genes and cell proliferation, migration and invasion of oral tongue squamous cell carcinoma (OTSCC). Additionally, we studied the effect of human tongue squamous carcinoma (HSC-3)-conditioned media on LINE-1 methylation and the expression of microenvironment remodeling genes in AT-MSCs and BM-MSCs. Conditioned media from HSC-3 or MSCs did not affect LINE-1 methylation level in either cancer cells or MSCs, respectively. In HSC-3 cells, no effect of MSCs-conditioned media was detected on the expression ofICAM1, ITGA3orMMP1. On the other hand, HSC-3-conditioned media upregulatedICAM1andMMP1expression in both types of MSCs. Co-cultures of AT-MSCs with HSC-3 did not induce proliferation, migration or invasion of the cancer cells. In conclusion, AT-MSCs, unlike BM-MSCs, seem not to participate in oral cancer progression.
Subject: mesenchymal stem cells
adipose tissue
bone marrow
oral squamous cell carcinoma
invasion
migration
MATRIX-METALLOPROTEINASE 1
BONE-MARROW
IN-VITRO
TUMOR
PROLIFERATION
HYPOMETHYLATION
PROMOTES
BREAST
MEDIA
LINES
1182 Biochemistry, cell and molecular biology
313 Dentistry
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