Development and validation of nucleic acid tests to diagnose Aleutian mink disease virus

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http://hdl.handle.net/10138/321263

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Virtanen , J , Aaltonen , K , Vapalahti , O & Sironen , T 2020 , ' Development and validation of nucleic acid tests to diagnose Aleutian mink disease virus ' , Journal of Virological Methods , vol. 279 , 113776 . https://doi.org/10.1016/j.jviromet.2019.113776

Title: Development and validation of nucleic acid tests to diagnose Aleutian mink disease virus
Author: Virtanen, Jenni; Aaltonen, Kirsi; Vapalahti, Olli; Sironen, Tarja
Contributor: University of Helsinki, Veterinary Biosciences
University of Helsinki, Veterinary Microbiology and Epidemiology
University of Helsinki, Department of Virology
University of Helsinki, Department of Virology
Date: 2020-05
Language: eng
Number of pages: 6
Belongs to series: Journal of Virological Methods
ISSN: 0166-0934
URI: http://hdl.handle.net/10138/321263
Abstract: Aleutian disease (AD), caused by Aleutian mink disease virus (AMDV), causes significant welfare problems to mink, and financial losses to the farmers. As there is no vaccine or treatment available, reliable diagnostics is important for disease control. Here, we set up a probe-based real-time PCR (NS1-probe-PCR) to detect all strains of AMDV. PCR was validated and compared to two other real-time PCR methods (pan-AMDV- and pan-AMDO-PCR) currently used for AMDV diagnostics in Finland. The NS1-probe-PCR had a similar detection limit of 20 copies/reaction based on plasmid dilution series, and similar or better diagnostic sensitivity, when evaluated using spleen samples from mink, and stool samples from mink and foxes. None of the three PCR tests cross-reacted with other parvoviruses. The NS1-probe-PCR also showed a significantly higher specificity than the pan-AMDO-PCR with spleen samples and the best specificity with stool samples. Furthermore, it produced the results more rapidly than the other two PCRs making it a promising tool for both diagnostic and research purposes.
Subject: AMDV
diagnostics
qPCR
NUCLEOTIDE-SEQUENCE
PARTIAL PROTECTION
PARVOVIRUS
TRANSMISSION
VACCINATION
ANTIBODIES
VIROLOGY
PROTEINS
STRAIN
11832 Microbiology and virology
413 Veterinary science
1182 Biochemistry, cell and molecular biology
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