Novel carbon film induces precocious calcium oscillation to promote neuronal cell maturation

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Ludwig , A , Kesaf , S , Heikkinen , J , Sukhanova , T , Khakipoor , S , Molinari , F , Pellegrino , C , Kim , S I , Han , J G , Huttunen , H J , Lauri , S E , Franssila , S , Jokinen , V & Rivera , C 2020 , ' Novel carbon film induces precocious calcium oscillation to promote neuronal cell maturation ' , Scientific Reports , vol. 10 , no. 1 , 17661 . https://doi.org/10.1038/s41598-020-74535-6

Title: Novel carbon film induces precocious calcium oscillation to promote neuronal cell maturation
Author: Ludwig, Anastasia; Kesaf, Sebnem; Heikkinen, Joonas; Sukhanova, Tatiana; Khakipoor, Shokoufeh; Molinari, Florence; Pellegrino, Christophe; Kim, Sung I.; Han, Jeon G.; Huttunen, Henri J.; Lauri, Sari E.; Franssila, Sami; Jokinen, Ville; Rivera, Claudio
Other contributor: University of Helsinki, Faculty Common Matters
University of Helsinki, Syn­aptic Plas­ti­city and De­vel­op­ment
University of Helsinki, Biosciences
University of Helsinki, Physiology and Neuroscience (-2020)
University of Helsinki, Division of Pharmaceutical Chemistry and Technology
University of Helsinki, Helsinki In Vivo Animal Imaging Platform (HAIP)









Date: 2020-10-19
Language: eng
Number of pages: 9
Belongs to series: Scientific Reports
ISSN: 2045-2322
DOI: https://doi.org/10.1038/s41598-020-74535-6
URI: http://hdl.handle.net/10138/321277
Abstract: Different types of carbon materials are biocompatible with neural cells and can promote maturation. The mechanism of this effect is not clear. Here we have tested the capacity of a carbon material composed of amorphous sp3 carbon backbone, embedded with a percolating network of sp2 carbon domains to sustain neuronal cultures. We found that cortical neurons survive and develop faster on this novel carbon material. After 3 days in culture, there is a precocious increase in the frequency of neuronal activity and in the expression of maturation marker KCC2 on carbon films as compared to a commonly used glass surface. Accelerated development is accompanied by a dramatic increase in neuronal dendrite arborization. The mechanism for the precocious maturation involves the activation of intracellular calcium oscillations by the carbon material already after 1 day in culture. Carbon-induced oscillations are independent of network activity and reflect intrinsic spontaneous activation of developing neurons. Thus, these results reveal a novel mechanism for carbon material-induced neuronal survival and maturation.
Subject: 3112 Neurosciences
1184 Genetics, developmental biology, physiology
NEURAL STEM-CELLS
DIFFERENTIATION
NANOMATERIALS
STIMULATION
NANOTUBES
FREQUENCY
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