Novel carbon film induces precocious calcium oscillation to promote neuronal cell maturation

Show simple item record Ludwig, Anastasia Kesaf, Sebnem Heikkinen, Joonas Sukhanova, Tatiana Khakipoor, Shokoufeh Molinari, Florence Pellegrino, Christophe Kim, Sung I. Han, Jeon G. Huttunen, Henri J. Lauri, Sari E. Franssila, Sami Jokinen, Ville Rivera, Claudio 2020-11-11T06:50:01Z 2020-11-11T06:50:01Z 2020-10-19
dc.identifier.citation Ludwig , A , Kesaf , S , Heikkinen , J , Sukhanova , T , Khakipoor , S , Molinari , F , Pellegrino , C , Kim , S I , Han , J G , Huttunen , H J , Lauri , S E , Franssila , S , Jokinen , V & Rivera , C 2020 , ' Novel carbon film induces precocious calcium oscillation to promote neuronal cell maturation ' , Scientific Reports , vol. 10 , no. 1 , 17661 .
dc.identifier.other PURE: 150286025
dc.identifier.other PURE UUID: 2855b123-278c-4ce3-bfac-490a18a9e3e5
dc.identifier.other RIS: urn:5D1D7D9813F31E9C6D114003729FE1D6
dc.identifier.other RIS: Ludwig2020
dc.identifier.other ORCID: /0000-0002-5895-1357/work/83433872
dc.identifier.other ORCID: /0000-0003-4849-8074/work/83433958
dc.identifier.other ORCID: /0000-0002-9867-4438/work/83434366
dc.identifier.other WOS: 000585195500011
dc.description.abstract Different types of carbon materials are biocompatible with neural cells and can promote maturation. The mechanism of this effect is not clear. Here we have tested the capacity of a carbon material composed of amorphous sp3 carbon backbone, embedded with a percolating network of sp2 carbon domains to sustain neuronal cultures. We found that cortical neurons survive and develop faster on this novel carbon material. After 3 days in culture, there is a precocious increase in the frequency of neuronal activity and in the expression of maturation marker KCC2 on carbon films as compared to a commonly used glass surface. Accelerated development is accompanied by a dramatic increase in neuronal dendrite arborization. The mechanism for the precocious maturation involves the activation of intracellular calcium oscillations by the carbon material already after 1 day in culture. Carbon-induced oscillations are independent of network activity and reflect intrinsic spontaneous activation of developing neurons. Thus, these results reveal a novel mechanism for carbon material-induced neuronal survival and maturation. en
dc.format.extent 9
dc.language.iso eng
dc.relation.ispartof Scientific Reports
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject 3112 Neurosciences
dc.subject 1184 Genetics, developmental biology, physiology
dc.subject STIMULATION
dc.subject NANOTUBES
dc.subject FREQUENCY
dc.title Novel carbon film induces precocious calcium oscillation to promote neuronal cell maturation en
dc.type Article
dc.contributor.organization Faculty Common Matters (Faculty of Biology and Environmental Sciences)
dc.contributor.organization Neuroscience Center
dc.contributor.organization Syn­aptic Plas­ti­city and De­vel­op­ment
dc.contributor.organization Biosciences
dc.contributor.organization Henri Juhani Huttunen / Principal Investigator
dc.contributor.organization Physiology and Neuroscience (-2020)
dc.contributor.organization Molecular and Integrative Biosciences Research Programme
dc.contributor.organization Division of Pharmaceutical Chemistry and Technology
dc.contributor.organization Helsinki In Vivo Animal Imaging Platform (HAIP)
dc.contributor.organization Claudio Rivera Baeza / Principal Investigator
dc.description.reviewstatus Peer reviewed
dc.relation.issn 2045-2322
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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