Pathogenicity of Campylobacter strains of poultry and human origin from Poland

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Wysok , B , Wojtacka , J & Kivistö , R 2020 , ' Pathogenicity of Campylobacter strains of poultry and human origin from Poland ' , International Journal of Food Microbiology , vol. 334 , 108830 . https://doi.org/10.1016/j.ijfoodmicro.2020.108830

Title: Pathogenicity of Campylobacter strains of poultry and human origin from Poland
Author: Wysok, Beata; Wojtacka, Joanna; Kivistö, Rauni
Other contributor: University of Helsinki, Food Hygiene and Environmental Health


Date: 2020-12-02
Language: eng
Number of pages: 10
Belongs to series: International Journal of Food Microbiology
ISSN: 0168-1605
DOI: https://doi.org/10.1016/j.ijfoodmicro.2020.108830
URI: http://hdl.handle.net/10138/321417
Abstract: The aim of this study was to determine the pathogenic markers associated with Campylobacter infection in humans. A total of 104 Campylobacter isolates obtained from poultry and humans were examined for the presence of nine virulence genes and their ability to adhere to, invade and produce cytotoxin were defined using HeLa cells. The diversity of the Campylobacter spp. isolates was studied based on sequencing of the SVR-region of flaA gene. Altogether 45 flaA-SVR alleles were identified among 104 Campylobacter isolates of poultry and human origin. All Campylobacter isolates possessed flaA, cadF and racR genes involved in adherence. Accordingly, all poultry and human isolates exhibited adherence towards HeLa cells at mean levels of 0.95% and 0.82% of starting viable inoculum, respectively. The genes involved in invasion (iam and pldA) and cytotoxin production (cdtA, cdtB and cdtC) were also widely distributed among the human and poultry Campylobacter isolates. Significantly higher invasiveness was observed for poultry isolates (mean level of 0.002% of starting bacterial inoculum) compared to human isolates (0.0005%). Interestingly the iam gene, associated with invasion, was more common in human (100%) than poultry (84%) isolates, and the poultry isolates lacking the iam gene showed a marked reduction in their ability to invade HeLa cells. Moreover, virB11 was present in 22% of the poultry and 70.4% of the human isolates. Strains lacking virB11 showed a slight reduction in invasion, however in the absence of iam even the poultry isolates containing virB11 were unable to invade HeLa cells. The mean cytotoxicity of Campylobacter isolates from poultry and human was 26.7% and 38.7%, respectively. Strains missing both the cdtB and cdtC genes were non-cytotoxic compared to strains containing all three cdtABC genes, which were the most cytotoxic among the C. jejuni and C. coli isolates from both sources. No cytotoxic effect was observed in only 4% of poultry and 5.6% of human isolates.
Subject: 416 Food Science
11832 Microbiology and virology
Campylobacter
Cytotoxicity
Adhesion
Invasion
Virulence factor
Cell line
flaA-SVR sequencing
DISTENDING TOXIN PRODUCTION
VIRULENCE GENES
MOLECULAR CHARACTERIZATION
JEJUNI
PREVALENCE
SPP.
PCR
IDENTIFICATION
CARCASSES
INVASION
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