No Correlation Between Nasopharyngeal Human Bocavirus 1 Genome Load and mRNA Detection or Serology in Adeno-/Tonsillectomy Patients

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Ivaska , L E , Christensen , A , Waris , M , Puhakka , T , Vuorinen , T , Allander , T , Söderlund-Venermo , M & Jartti , T 2019 , ' No Correlation Between Nasopharyngeal Human Bocavirus 1 Genome Load and mRNA Detection or Serology in Adeno-/Tonsillectomy Patients ' , Journal of Infectious Diseases , vol. 220 , no. 4 , pp. 589-593 . https://doi.org/10.1093/infdis/jiz166

Title: No Correlation Between Nasopharyngeal Human Bocavirus 1 Genome Load and mRNA Detection or Serology in Adeno-/Tonsillectomy Patients
Author: Ivaska, Lotta E.; Christensen, Andreas; Waris, Matti; Puhakka, Tuomo; Vuorinen, Tytti; Allander, Tobias; Söderlund-Venermo, Maria; Jartti, Tuomas
Contributor organization: Human Parvoviruses: Epidemiology, Molecular Biology and Clinical Impact
Department of Virology
Medicum
University of Helsinki
Date: 2019-08-15
Language: eng
Number of pages: 5
Belongs to series: Journal of Infectious Diseases
ISSN: 0022-1899
DOI: https://doi.org/10.1093/infdis/jiz166
URI: http://hdl.handle.net/10138/321529
Abstract: Human bocavirus 1 (HBoV1) can persist in nasopharynx and tonsils. Using HBoV1 serology, reverse-transcription polymerase chain reaction (PCR) for detecting messenger RNA (mRNA) and quantitative PCR for HBoV1 genome load count, we studied to what extent the HBoV1 DNA loads in nasopharynx correlate with acute infection markers. Tonsillar tissue, nasopharyngeal aspirate, and serum were obtained from 188 elective adeno-/tonsillectomy patients. Relatively high loads of HBoV1 DNA were detected in the nasopharynx of 14 (7%) primarily asymptomatic subjects with negative mRNA and/or serodiagnostic results. Quantitative HBoV1 DNA PCR may have lower specificity than HBoV1 mRNA detection for diagnosing symptomatic infection.
Subject: bocavirus
parvovirus
nasopharynx
tonsil
serology
diagnosis
detection
CHILDREN
3111 Biomedicine
Peer reviewed: Yes
Rights: unspecified
Usage restriction: openAccess
Self-archived version: acceptedVersion


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