Substrate specificities of inteins investigated by QuickDrop-cassette mutagenesis

Show full item record



Permalink

http://hdl.handle.net/10138/321547

Citation

Oeemig , J S , Beyer , H M , Aranko , A S , Mutanen , J & Iwai , H 2020 , ' Substrate specificities of inteins investigated by QuickDrop-cassette mutagenesis ' , FEBS Letters , vol. 594 , no. 20 , pp. 3338-3355 . https://doi.org/10.1002/1873-3468.13909

Title: Substrate specificities of inteins investigated by QuickDrop-cassette mutagenesis
Author: Oeemig, Jesper S.; Beyer, Hannes M.; Aranko, A. Sesilja; Mutanen, Justus; Iwai, Hideo
Contributor: University of Helsinki, Institute of Biotechnology
University of Helsinki, Institute of Biotechnology
University of Helsinki, Institute of Biotechnology
University of Helsinki, Maker@STEAM
University of Helsinki, Institute of Biotechnology
Date: 2020-10
Language: eng
Number of pages: 18
Belongs to series: FEBS Letters
ISSN: 0014-5793
URI: http://hdl.handle.net/10138/321547
Abstract: Inteins catalyze self-excision from host precursor proteins while concomitantly ligating the flanking substrates (exteins) with a peptide bond. Noncatalytic extein residues near the splice junctions, such as the residues at the -1 and +2 positions, often strongly influence the protein-splicing efficiency. The substrate specificities of inteins have not been studied for many inteins. We developed a convenient mutagenesis platform termed "QuickDrop"-cassette mutagenesis for investigating the influences of 20 amino acid types at the -1 and +2 positions of different inteins. We elucidated 17 different profiles of the 20 amino acid dependencies across different inteins. The substrate specificities will accelerate our understanding of the structure-function relationship at the splicing junctions for broader applications of inteins in biotechnology and molecular biosciences.
Subject: intein
mutagenesis
protein ligation
protein splicing
substrate specificity
HIGHLY EFFICIENT
SPLIT INTEINS
DNAE INTEIN
PROTEIN
ENDONUCLEASES
PURIFICATION
LIGATION
AMINO
NMR
CIS
1182 Biochemistry, cell and molecular biology
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
1873_3468.13909.pdf 3.381Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record