Substrate specificities of inteins investigated by QuickDrop-cassette mutagenesis

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dc.contributor.author Oeemig, Jesper S.
dc.contributor.author Beyer, Hannes M.
dc.contributor.author Aranko, A. Sesilja
dc.contributor.author Mutanen, Justus
dc.contributor.author Iwai, Hideo
dc.date.accessioned 2020-11-16T13:02:01Z
dc.date.available 2020-11-16T13:02:01Z
dc.date.issued 2020-10
dc.identifier.citation Oeemig , J S , Beyer , H M , Aranko , A S , Mutanen , J & Iwai , H 2020 , ' Substrate specificities of inteins investigated by QuickDrop-cassette mutagenesis ' , FEBS Letters , vol. 594 , no. 20 , pp. 3338-3355 . https://doi.org/10.1002/1873-3468.13909
dc.identifier.other PURE: 151244162
dc.identifier.other PURE UUID: c5dc217f-9fe3-40e6-82eb-6890b3c046e6
dc.identifier.other WOS: 000572691100001
dc.identifier.other ORCID: /0000-0001-7376-5264/work/83837816
dc.identifier.other ORCID: /0000-0001-9023-2101/work/83841564
dc.identifier.other ORCID: /0000-0002-6882-006X/work/83842827
dc.identifier.uri http://hdl.handle.net/10138/321547
dc.description.abstract Inteins catalyze self-excision from host precursor proteins while concomitantly ligating the flanking substrates (exteins) with a peptide bond. Noncatalytic extein residues near the splice junctions, such as the residues at the -1 and +2 positions, often strongly influence the protein-splicing efficiency. The substrate specificities of inteins have not been studied for many inteins. We developed a convenient mutagenesis platform termed "QuickDrop"-cassette mutagenesis for investigating the influences of 20 amino acid types at the -1 and +2 positions of different inteins. We elucidated 17 different profiles of the 20 amino acid dependencies across different inteins. The substrate specificities will accelerate our understanding of the structure-function relationship at the splicing junctions for broader applications of inteins in biotechnology and molecular biosciences. en
dc.format.extent 18
dc.language.iso eng
dc.relation.ispartof FEBS Letters
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject intein
dc.subject mutagenesis
dc.subject protein ligation
dc.subject protein splicing
dc.subject substrate specificity
dc.subject HIGHLY EFFICIENT
dc.subject SPLIT INTEINS
dc.subject DNAE INTEIN
dc.subject PROTEIN
dc.subject ENDONUCLEASES
dc.subject PURIFICATION
dc.subject LIGATION
dc.subject AMINO
dc.subject NMR
dc.subject CIS
dc.subject 1182 Biochemistry, cell and molecular biology
dc.title Substrate specificities of inteins investigated by QuickDrop-cassette mutagenesis en
dc.type Article
dc.contributor.organization Institute of Biotechnology
dc.contributor.organization Maker@STEAM
dc.contributor.organization Biosciences
dc.contributor.organization University Management
dc.contributor.organization Doctoral Programme in Drug Research
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1002/1873-3468.13909
dc.relation.issn 0014-5793
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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