Combined Effect of Naturally-Derived Biofilm Inhibitors and Differentiated HL-60 Cells in the Prevention of Staphylococcus aureus Biofilm Formation

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Reigada , I , Guarch-Pérez , C M , Patel , J , Riool , M , Savijoki , K , Yli-Kauhaluoma , J , Zaat , S A J & Fallarero , A 2020 , ' Combined Effect of Naturally-Derived Biofilm Inhibitors and Differentiated HL-60 Cells in the Prevention of Staphylococcus aureus Biofilm Formation ' , Microorganisms , vol. 8 , no. 11 , 1757 . https://doi.org/10.3390/microorganisms8111757

Title: Combined Effect of Naturally-Derived Biofilm Inhibitors and Differentiated HL-60 Cells in the Prevention of Staphylococcus aureus Biofilm Formation
Author: Reigada, Inés; Guarch-Pérez, Clara M.; Patel, Jayendra; Riool, Martijn; Savijoki, Kirsi; Yli-Kauhaluoma, Jari; Zaat, Sebastian A. J.; Fallarero, Adyary
Contributor: University of Helsinki, Pharmaceutical Design and Discovery group
University of Helsinki, Pharmaceutical Design and Discovery group
University of Helsinki, Division of Pharmaceutical Biosciences
University of Helsinki, Pharmaceutical Design and Discovery group
University of Helsinki, Divisions of Faculty of Pharmacy
Date: 2020-11-09
Language: eng
Number of pages: 15
Belongs to series: Microorganisms
ISSN: 2076-2607
URI: http://hdl.handle.net/10138/321910
Abstract: Nosocomial diseases represent a huge health and economic burden. A significant portion is associated with the use of medical devices, with 80% of these infections being caused by a bacterial biofilm. The insertion of a foreign material usually elicits inflammation, which can result in hampered antimicrobial capacity of the host immunity due to the effort of immune cells being directed to degrade the material. The ineffective clearance by immune cells is a perfect opportunity for bacteria to attach and form a biofilm. In this study, we analyzed the antibiofilm capacity of three naturally derived biofilm inhibitors when combined with immune cells in order to assess their applicability in implantable titanium devices and low-density polyethylene (LDPE) endotracheal tubes. To this end, we used a system based on the coculture of HL-60 cells differentiated into polymorphonuclear leukocytes (PMNs) and Staphylococcus aureus (laboratory and clinical strains) on titanium, as well as LDPE surfaces. Out of the three inhibitors, the one coded DHA1 showed the highest potential to be incorporated into implantable devices, as it displayed a combined activity with the immune cells, preventing bacterial attachment on the titanium and LDPE. The other two inhibitors seemed to also be good candidates for incorporation into LDPE endotracheal tubes.
Subject: 317 Pharmacy
Staphylococcus aureus
biomaterials
medical devices
HL-60 cells
PMNs
biofilm
endotracheal tube
titanium
implantable devices
nosocomial diseases
INFECTIONS
DIAGNOSIS
IMPLANTS
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