Heterochronic Developmental Shifts Underlying Squamate Cerebellar Diversity Unveil the Key Features of Amniote Cerebellogenesis

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Macrì , S & Di-Poï , N 2020 , ' Heterochronic Developmental Shifts Underlying Squamate Cerebellar Diversity Unveil the Key Features of Amniote Cerebellogenesis ' , Frontiers in Cell and Developmental Biology , vol. 8 , 593377 . https://doi.org/10.3389/fcell.2020.593377

Title: Heterochronic Developmental Shifts Underlying Squamate Cerebellar Diversity Unveil the Key Features of Amniote Cerebellogenesis
Author: Macrì, Simone; Di-Poï, Nicolas
Contributor: University of Helsinki, Institute of Biotechnology
University of Helsinki, Institute of Biotechnology
Date: 2020-10-22
Language: eng
Number of pages: 22
Belongs to series: Frontiers in Cell and Developmental Biology
ISSN: 2296-634X
URI: http://hdl.handle.net/10138/321956
Abstract: Despite a remarkable conservation of architecture and function, the cerebellum of vertebrates shows extensive variation in morphology, size, and foliation pattern. These features make this brain subdivision a powerful model to investigate the evolutionary developmental mechanisms underlying neuroanatomical complexity both within and between anamniote and amniote species. Here, we fill a major evolutionary gap by characterizing the developing cerebellum in two non-avian reptile species—bearded dragon lizard and African house snake—representative of extreme cerebellar morphologies and neuronal arrangement patterns found in squamates. Our data suggest that developmental strategies regarded as exclusive hallmark of birds and mammals, including transit amplification in an external granule layer (EGL) and Sonic hedgehog expression by underlying Purkinje cells (PCs), contribute to squamate cerebellogenesis independently from foliation pattern. Furthermore, direct comparison of our models suggests the key importance of spatiotemporal patterning and dynamic interaction between granule cells and PCs in defining cortical organization. Especially, the observed heterochronic shifts in early cerebellogenesis events, including upper rhombic lip progenitor activity and EGL maintenance, are strongly expected to affect the dynamics of molecular interaction between neuronal cell types in snakes. Altogether, these findings help clarifying some of the morphogenetic and molecular underpinnings of amniote cerebellar corticogenesis, but also suggest new potential molecular mechanisms underlying cerebellar complexity in squamates. Furthermore, squamate models analyzed here are revealed as key animal models to further understand mechanisms of brain organization.
Subject: 1182 Biochemistry, cell and molecular biology
development
evolution
cerebellum
squamates
patterning
PURKINJE-CELL REPLACEMENT
REELER MUTANT MOUSE
RECEPTOR ROR-ALPHA
SONIC HEDGEHOG
RHOMBIC-LIP
QUANTITATIVE-ANALYSIS
NEURON PRECURSORS
CHOROID-PLEXUS
GRANULE CELLS
ZEBRIN-II
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