Cyclooxygenase-2 is inhibited in prolonged luteal maintenance induced by intrauterine devices in mares

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http://hdl.handle.net/10138/322238

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Montserrat Rivera del Alamo , M , Reilas , T , Galvao , A , Yeste , M & Katila , T 2018 , ' Cyclooxygenase-2 is inhibited in prolonged luteal maintenance induced by intrauterine devices in mares ' , Animal Reproduction Science , vol. 199 , pp. 93-103 . https://doi.org/10.1016/j.anireprosci.2018.11.004

Title: Cyclooxygenase-2 is inhibited in prolonged luteal maintenance induced by intrauterine devices in mares
Author: Montserrat Rivera del Alamo, Maria; Reilas, Tiina; Galvao, Antonio; Yeste, Marc; Katila, Terttu
Other contributor: University of Helsinki, Departments of Faculty of Veterinary Medicine


Date: 2018-12
Language: eng
Number of pages: 11
Belongs to series: Animal Reproduction Science
ISSN: 0378-4320
DOI: https://doi.org/10.1016/j.anireprosci.2018.11.004
URI: http://hdl.handle.net/10138/322238
Abstract: Treatment with intrauterine devices (IUD) prolongs luteal phases in mares, but the mechanism for this has not been fully elucidated. The aims of the present study were to examine how IUDs affect the uterus to induce longer luteal phases, particularly the role of cyclooxygenase-2 (COX-2) in the maintenance of the corpus luteum (CL). Twenty-seven reproductively normal mares were included: 12 were inseminated (AI), and 15 were fitted with IUDs. Blood samples for progesterone were obtained on Days 0, 3, 5, 7, 9, 11, 13, 14, and 15 (relative to day of ovulation). The groups were further divided into non-pregnant (AI-N, n = 4), pregnant (AI-P, n = 8), normal (IUD-N, n = 8) and prolonged luteal phase (IUD-P, n = 7) based on ultrasonic examinations and serum progesterone concentrations on Days 14 and 15. Blood sampling to quantify the PGF(2 alpha) metabolite (PGFM) was performed through a catheter hourly from 15:00 to 20:00 h on Day 14, and from 6:00 until 13:00 h on Day 15. On Day 15, a low-volume uterine lavage followed by an endometrial biopsy was performed. Estradiol concentration in the Day 15 serum and lavage fluid was determined, while the abundance of COX-2 was evaluated in the biopsy specimens using western blotting (WB) and irnmunohistochemistry (IHC). All pregnant mares were negative for COX-2 in IHC samples and 5 of 8 were negative in WB samples while all mares of the IUD-N group were positive for COX-2. Of the seven mares in the IUD-P group, five and four were negative for COX-2 with the IHC and WB samples, respectively. The results from this study indicate that IUDs, when effective, suppress COX-2, leading to the inhibition of PGF2 alpha release and maintenance of CL.
Subject: Cyclooxygenase-2
PGF2 alpha
Progesterone
Inflammation
Intrauterine device
Horse
PERSISTENT CORPUS-LUTEUM
ESTROUS-CYCLE
UTERINE INFLAMMATION
EARLY-PREGNANCY
SEMINAL PLASMA
EXPRESSION
PGF2-ALPHA
ESTRADIOL
PROGESTERONE
ENDOMETRIUM
413 Veterinary science
412 Animal science, dairy science
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