Cyclooxygenase-2 is inhibited in prolonged luteal maintenance induced by intrauterine devices in mares

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dc.contributor.author Montserrat Rivera del Alamo, Maria
dc.contributor.author Reilas, Tiina
dc.contributor.author Galvao, Antonio
dc.contributor.author Yeste, Marc
dc.contributor.author Katila, Terttu
dc.date.accessioned 2020-12-01T11:38:01Z
dc.date.available 2020-12-01T11:38:01Z
dc.date.issued 2018-12
dc.identifier.citation Montserrat Rivera del Alamo , M , Reilas , T , Galvao , A , Yeste , M & Katila , T 2018 , ' Cyclooxygenase-2 is inhibited in prolonged luteal maintenance induced by intrauterine devices in mares ' , Animal Reproduction Science , vol. 199 , pp. 93-103 . https://doi.org/10.1016/j.anireprosci.2018.11.004
dc.identifier.other PURE: 121714193
dc.identifier.other PURE UUID: 7acbeeac-bc2e-4603-9561-f952e91414ce
dc.identifier.other WOS: 000454969300012
dc.identifier.other Scopus: 85056643143
dc.identifier.uri http://hdl.handle.net/10138/322238
dc.description.abstract Treatment with intrauterine devices (IUD) prolongs luteal phases in mares, but the mechanism for this has not been fully elucidated. The aims of the present study were to examine how IUDs affect the uterus to induce longer luteal phases, particularly the role of cyclooxygenase-2 (COX-2) in the maintenance of the corpus luteum (CL). Twenty-seven reproductively normal mares were included: 12 were inseminated (AI), and 15 were fitted with IUDs. Blood samples for progesterone were obtained on Days 0, 3, 5, 7, 9, 11, 13, 14, and 15 (relative to day of ovulation). The groups were further divided into non-pregnant (AI-N, n = 4), pregnant (AI-P, n = 8), normal (IUD-N, n = 8) and prolonged luteal phase (IUD-P, n = 7) based on ultrasonic examinations and serum progesterone concentrations on Days 14 and 15. Blood sampling to quantify the PGF(2 alpha) metabolite (PGFM) was performed through a catheter hourly from 15:00 to 20:00 h on Day 14, and from 6:00 until 13:00 h on Day 15. On Day 15, a low-volume uterine lavage followed by an endometrial biopsy was performed. Estradiol concentration in the Day 15 serum and lavage fluid was determined, while the abundance of COX-2 was evaluated in the biopsy specimens using western blotting (WB) and irnmunohistochemistry (IHC). All pregnant mares were negative for COX-2 in IHC samples and 5 of 8 were negative in WB samples while all mares of the IUD-N group were positive for COX-2. Of the seven mares in the IUD-P group, five and four were negative for COX-2 with the IHC and WB samples, respectively. The results from this study indicate that IUDs, when effective, suppress COX-2, leading to the inhibition of PGF2 alpha release and maintenance of CL. en
dc.format.extent 11
dc.language.iso eng
dc.relation.ispartof Animal Reproduction Science
dc.rights cc_by_nc_nd
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject Cyclooxygenase-2
dc.subject PGF2 alpha
dc.subject Progesterone
dc.subject Inflammation
dc.subject Intrauterine device
dc.subject Horse
dc.subject PERSISTENT CORPUS-LUTEUM
dc.subject ESTROUS-CYCLE
dc.subject UTERINE INFLAMMATION
dc.subject EARLY-PREGNANCY
dc.subject SEMINAL PLASMA
dc.subject EXPRESSION
dc.subject PGF2-ALPHA
dc.subject ESTRADIOL
dc.subject PROGESTERONE
dc.subject ENDOMETRIUM
dc.subject 413 Veterinary science
dc.subject 412 Animal science, dairy science
dc.title Cyclooxygenase-2 is inhibited in prolonged luteal maintenance induced by intrauterine devices in mares en
dc.type Article
dc.contributor.organization Departments of Faculty of Veterinary Medicine
dc.contributor.organization Production Animal Medicine
dc.contributor.organization Faculty of Veterinary Medicine
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1016/j.anireprosci.2018.11.004
dc.relation.issn 0378-4320
dc.rights.accesslevel openAccess
dc.type.version acceptedVersion

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