Blood-based epigenetic estimators of chronological age in human adults using DNA methylation data from the Illumina MethylationEPIC array

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Lee , Y , Haftorn , K L , Denault , W R P , Nustad , H E , Page , C M , Lyle , R , Lee-Odegard , S , Moen , G-H , Prasad , R B , Groop , L C , Sletner , L , Sommer , C , Magnus , M C , Gjessing , H K , Harris , J R , Magnus , P , Haberg , S E , Jugessur , A & Bohlin , J 2020 , ' Blood-based epigenetic estimators of chronological age in human adults using DNA methylation data from the Illumina MethylationEPIC array ' , BMC Genomics , vol. 21 , no. 1 , 747 . https://doi.org/10.1186/s12864-020-07168-8

Title: Blood-based epigenetic estimators of chronological age in human adults using DNA methylation data from the Illumina MethylationEPIC array
Author: Lee, Yunsung; Haftorn, Kristine L.; Denault, William R. P.; Nustad, Haakon E.; Page, Christian M.; Lyle, Robert; Lee-Odegard, Sindre; Moen, Gunn-Helen; Prasad, Rashmi B.; Groop, Leif C.; Sletner, Line; Sommer, Christine; Magnus, Maria C.; Gjessing, Hakon K.; Harris, Jennifer R.; Magnus, Per; Haberg, Siri E.; Jugessur, Astanand; Bohlin, Jon
Contributor: University of Helsinki, Centre of Excellence in Complex Disease Genetics
Date: 2020-10-27
Language: eng
Number of pages: 13
Belongs to series: BMC Genomics
ISSN: 1471-2164
URI: http://hdl.handle.net/10138/322342
Abstract: BackgroundEpigenetic clocks have been recognized for their precise prediction of chronological age, age-related diseases, and all-cause mortality. Existing epigenetic clocks are based on CpGs from the Illumina HumanMethylation450 BeadChip (450K) which has now been replaced by the latest platform, Illumina MethylationEPIC BeadChip (EPIC). Thus, it remains unclear to what extent EPIC contributes to increased precision and accuracy in the prediction of chronological age.ResultsWe developed three blood-based epigenetic clocks for human adults using EPIC-based DNA methylation (DNAm) data from the Norwegian Mother, Father and Child Cohort Study (MoBa) and the Gene Expression Omnibus (GEO) public repository: 1) an Adult Blood-based EPIC Clock (ABEC) trained on DNAm data from MoBa (n=1592, age-span: 19 to 59years), 2) an extended ABEC (eABEC) trained on DNAm data from MoBa and GEO (n=2227, age-span: 18 to 88years), and 3) a common ABEC (cABEC) trained on the same training set as eABEC but restricted to CpGs common to 450K and EPIC. Our clocks showed high precision (Pearson correlation between chronological and epigenetic age (r)>0.94) in independent cohorts, including GSE111165 (n=15), GSE115278 (n=108), GSE132203 (n=795), and the Epigenetics in Pregnancy (EPIPREG) study of the STORK Groruddalen Cohort (n=470). This high precision is unlikely due to the use of EPIC, but rather due to the large sample size of the training set.ConclusionsOur ABECs predicted adults' chronological age precisely in independent cohorts. As EPIC is now the dominant platform for measuring DNAm, these clocks will be useful in further predictions of chronological age, age-related diseases, and mortality.
Subject: DNA methylation
Epigenetic age
Chronological age
Illumina MethylationEPIC BeadChip
MoBa
NORWEGIAN MOTHER
CHILD COHORT
REGULARIZATION
PROFILES
OBESITY
CANCER
CELLS
3111 Biomedicine
11832 Microbiology and virology
1184 Genetics, developmental biology, physiology
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