Cancer Alters the Metabolic Fingerprint of Extracellular Vesicles

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http://hdl.handle.net/10138/323148

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Palviainen , M , Laukkanen , K , Tavukcuoglu , Z , Velagapudi , V , Kärkkäinen , O , Hanhineva , K , Auriola , S , Ranki , A & Siljander , P 2020 , ' Cancer Alters the Metabolic Fingerprint of Extracellular Vesicles ' , Cancers , vol. 12 , no. 11 , 3292 . https://doi.org/10.3390/cancers12113292

Title: Cancer Alters the Metabolic Fingerprint of Extracellular Vesicles
Author: Palviainen, Mari; Laukkanen, Kirsi; Tavukcuoglu, Zeynep; Velagapudi, Vidya; Kärkkäinen, Olli; Hanhineva, Kati; Auriola, Seppo; Ranki, Annamari; Siljander, Pia
Contributor: University of Helsinki, Extracellular Vesicles
University of Helsinki, HUS Inflammation Center
University of Helsinki, Divisions of Faculty of Pharmacy
University of Helsinki, Institute for Molecular Medicine Finland
University of Helsinki, HUS Inflammation Center
University of Helsinki, Extracellular Vesicles
Date: 2020-11-06
Language: eng
Number of pages: 18
Belongs to series: Cancers
ISSN: 2072-6694
URI: http://hdl.handle.net/10138/323148
Abstract: Cancer alters cell metabolism. How these changes are manifested in the metabolite cargo of cancer-derived extracellular vesicles (EVs) remains poorly understood. To explore these changes, EVs from prostate, cutaneous T-cell lymphoma (CTCL), colon cancer cell lines, and control EVs from their noncancerous counterparts were isolated by differential ultracentrifugation and analyzed by nanoparticle tracking analysis (NTA), electron microscopy (EM), Western blotting, and liquid chromatography-mass spectrometry (LC-MS). Although minor differences between the cancerous and non-cancerous cell-derived EVs were observed by NTA and Western blotting, the largest differences were detected in their metabolite cargo. Compared to EVs from noncancerous cells, cancer EVs contained elevated levels of soluble metabolites, e.g., amino acids and B vitamins. Two metabolites, proline and succinate, were elevated in the EV samples of all three cancer types. In addition, folate and creatinine were elevated in the EVs from prostate and CTCL cancer cell lines. In conclusion, we present the first evidence in vitro that the altered metabolism of different cancer cells is reflected in common metabolite changes in their EVs. These results warrant further studies on the significance and usability of this metabolic fingerprint in cancer.
Subject: 1182 Biochemistry, cell and molecular biology
extracellular vesicles
cancer metabolism
cutaneous T-cell lymphoma
prostate cancer
colon cancer
ONE-CARBON METABOLISM
T-CELL LYMPHOMA
SERUM CREATININE
PROLINE METABOLISM
BIOMARKERS
HALLMARKS
RISK
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