Pectobacterium parvum sp. nov., having a Salmonella SPI-1-like Type III secretion system and low virulence

Show simple item record Pasanen, Miia Waleron, Malgorzata Schott, Thomas Cleenwerck, Ilse Misztak, Agnieszka Waleron, Krzysztof Pritchard, Leighton Bakr, Ramadan Degefu, Yeshitila van der Wolf, Jan Vandamme, Peter Pirhonen, Minna 2020-12-17T09:26:02Z 2020-12-17T09:26:02Z 2020
dc.identifier.citation Pasanen , M , Waleron , M , Schott , T , Cleenwerck , I , Misztak , A , Waleron , K , Pritchard , L , Bakr , R , Degefu , Y , van der Wolf , J , Vandamme , P & Pirhonen , M 2020 , ' Pectobacterium parvum sp. nov., having a Salmonella SPI-1-like Type III secretion system and low virulence ' , International Journal of Systematic and Evolutionary Microbiology , vol. 70 , no. 4 , pp. 2440-2448 . ,
dc.identifier.other PURE: 157651448
dc.identifier.other PURE UUID: 16727d66-e766-4f77-8226-cb4aa62183b7
dc.identifier.other WOS: 000584502400038
dc.identifier.other WOS: 000584502400098
dc.identifier.other ORCID: /0000-0001-5249-563X/work/85518647
dc.description Corrigendum: Pectobacterium parvum sp. nov., having a Salmonella SPI-1-like Type III secretion system and low virulence. Pages: 2959-2959.
dc.description.abstract Pectobacterium strains isolated from potato stems in Finland, Poland and the Netherlands were subjected to polyphasic analyses to characterize their genomic and phenotypic features. Phylogenetic analysis based on 382 core proteins showed that the isolates clustered closest to Pectobacterium polaris but could be divided into two clades. Average nucleotide identity (ANI) analysis revealed that the isolates in one of the clades included the P. polaris type strain, whereas the second Glade was at the border of the species P. polaris with a 96 % ANI value. In silico genome-to-genome comparisons between the isolates revealed values below 70%, patristic distances based on 1294 core proteins were at the level observed between closely related Pectobacterium species, and the two groups of bacteria differed in genome size, G+C content and results of amplified fragment length polymorphism and Biolog analyses. Comparisons between the genomes revealed that the isolates of the atypical group contained SPI-1-type Type III secretion island and genes coding for proteins known for toxic effects on nematodes or insects, and lacked many genes coding for previously characterized virulence determinants affecting rotting of plant tissue by soft rot bacteria. Furthermore, the atypical isolates could be differentiated from P. polaris by their low virulence, production of antibacterial metabolites and a citrate-negative phenotype. Based on the results of a polyphasic approach including genome-to-genome comparisons, biochemical and virulence assays, presented in this report, we propose delineation of the atypical isolates as a novel species Pectobacterium parvum, for which the isolate s0421(T) (CFBP 8630(T)=LMG 30828(T)) is suggested as a type strain. en
dc.format.extent 9
dc.language.iso eng
dc.relation.ispartof International Journal of Systematic and Evolutionary Microbiology
dc.rights cc_by_nc
dc.rights cc_by_nc
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject Pectobacterium
dc.subject Pectobacterium parvum
dc.subject Salmonella SPI-1 T3SS
dc.subject potato
dc.subject soft rot
dc.subject virulence
dc.subject POTATO
dc.subject CAROTOVORA
dc.subject WASABIAE
dc.subject SEQUENCE
dc.subject HOST
dc.subject GENE
dc.subject RESISTANCE
dc.subject NEMATODES
dc.subject 11832 Microbiology and virology
dc.title Pectobacterium parvum sp. nov., having a Salmonella SPI-1-like Type III secretion system and low virulence en
dc.type Article
dc.contributor.organization Faculty of Agriculture and Forestry
dc.contributor.organization Department of Agricultural Sciences
dc.contributor.organization Viikki Plant Science Centre (ViPS)
dc.contributor.organization Minna Pirhonen / Principal Investigator
dc.contributor.organization Plant Production Sciences
dc.description.reviewstatus Peer reviewed
dc.relation.issn 1466-5026
dc.rights.accesslevel openAccess
dc.type.version publishedVersion
dc.type.version publishedVersion

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