Genetic Adaptation of Coxsackievirus B1 during Persistent Infection in Pancreatic Cells

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Honkimaa , A , Kimura , B , Sioofy-Khojine , A-B , Lin , J , Laiho , J , Oikarinen , S & Hyöty , H 2020 , ' Genetic Adaptation of Coxsackievirus B1 during Persistent Infection in Pancreatic Cells ' , Microorganisms , vol. 8 , no. 11 , 1790 . https://doi.org/10.3390/microorganisms8111790

Title: Genetic Adaptation of Coxsackievirus B1 during Persistent Infection in Pancreatic Cells
Author: Honkimaa, Anni; Kimura, Bryn; Sioofy-Khojine, Amir-Babak; Lin, Jake; Laiho, Jutta; Oikarinen, Sami; Hyöty, Heikki
Contributor organization: Complex Disease Genetics
Statistical and population genetics
Institute for Molecular Medicine Finland
Date: 2020-11
Language: eng
Number of pages: 21
Belongs to series: Microorganisms
ISSN: 2076-2607
DOI: https://doi.org/10.3390/microorganisms8111790
URI: http://hdl.handle.net/10138/323504
Abstract: Coxsackie B (CVB) viruses have been associated with type 1 diabetes. We have recently observed that CVB1 was linked to the initiation of the autoimmune process leading to type 1 diabetes in Finnish children. Viral persistency in the pancreas is currently considered as one possible mechanism. In the current study persistent infection was established in pancreatic ductal and beta cell lines (PANC-1 and 1.1B4) using four different CVB1 strains, including the prototype strain and three clinical isolates. We sequenced 5 ' untranslated region (UTR) and regions coding for structural and non-structural proteins and the second single open reading frame (ORF) protein of all persisting CVB1 strains using next generation sequencing to identify mutations that are common for all of these strains. One mutation, K257R in VP1, was found from all persisting CVB1 strains. The mutations were mainly accumulated in viral structural proteins, especially at BC, DE, EF loops and C-terminus of viral capsid protein 1 (VP1), the puff region of VP2, the knob region of VP3 and infection-enhancing epitope of VP4. This showed that the capsid region of the viruses sustains various changes during persistency some of which could be hallmark(s) of persistency.
Subject: type 1 diabetes
enterovirus
coxsackievirus B1
next generation sequencing
persistent infection
cell models of persistency
virus adaptation
DECAY-ACCELERATING FACTOR
AMINO-ACID SUBSTITUTION
ADENOVIRUS RECEPTOR
MONOCLONAL-ANTIBODIES
POINT MUTATION
VP1 PROTEIN
PUFF REGION
FACTOR DAF
B3
ENTEROVIRUS
11832 Microbiology and virology
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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