IMPROvER : the Integral Membrane Protein Stability Selector

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Harborne , S P D , Strauss , J , Boakes , J C , Wright , D L , Henderson , J G , Boivineau , J , Jaakola , V-P & Goldman , A 2020 , ' IMPROvER : the Integral Membrane Protein Stability Selector ' , Scientific Reports , vol. 10 , no. 1 , 15165 . https://doi.org/10.1038/s41598-020-71744-x

Title: IMPROvER : the Integral Membrane Protein Stability Selector
Author: Harborne, Steven P. D.; Strauss, Jannik; Boakes, Jessica C.; Wright, Danielle L.; Henderson, James G.; Boivineau, Jacques; Jaakola, Veli-Pekka; Goldman, Adrian
Contributor: University of Helsinki, Biochemistry and Biotechnology
Date: 2020-09-16
Language: eng
Number of pages: 18
Belongs to series: Scientific Reports
ISSN: 2045-2322
URI: http://hdl.handle.net/10138/323506
Abstract: Identifying stabilising variants of membrane protein targets is often required for structure determination. Our new computational pipeline, the Integral Membrane Protein Stability Selector (IMPROvER) provides a rational approach to variant selection by employing three independent approaches: deep-sequence, model-based and data-driven. In silico tests using known stability data, and in vitro tests using three membrane protein targets with 7, 11 and 16 transmembrane helices provided measures of success. In vitro, individual approaches alone all identified stabilising variants at a rate better than expected by random selection. Low numbers of overlapping predictions between approaches meant a greater success rate was achieved (fourfold better than random) when approaches were combined and selections restricted to the highest ranked sites. The mix of information IMPROvER uses can be extracted for any helical membrane protein. We have developed the first general-purpose tool for selecting stabilising variants of alpha -helical membrane proteins, increasing efficiency and reducing workload. IMPROvER can be accessed at http://improver.ddns.net/IMPROvER/.
Subject: EQUILIBRATIVE NUCLEOSIDE TRANSPORTER
VACUOLAR H+-PYROPHOSPHATASE
AMINO-ACID-RESIDUES
THERMOSTABILIZING MUTATIONS
FUNCTIONAL-CHARACTERIZATION
BOUND PYROPHOSPHATASES
IDENTIFICATION
RECEPTOR
ADENOSINE
HENT1
1182 Biochemistry, cell and molecular biology
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