Molecular diversity and phytochemical variability in the Iranian poppy (Papaver bracteatum Lindl.): A baseline for conservation and utilization in future breeding programmes

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http://hdl.handle.net/10138/323866

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Qaderi , A , Omidi , M , Pour-Aboughadareh , A , Poczai , P , Shaghaghi , J , MehrAfarin , A , Nohooji , M G & Etminan , A 2019 , ' Molecular diversity and phytochemical variability in the Iranian poppy (Papaver bracteatum Lindl.): A baseline for conservation and utilization in future breeding programmes ' , Industrial Crops and Products , vol. 130 , pp. 237-247 . https://doi.org/10.1016/j.indcrop.2018.12.079

Titel: Molecular diversity and phytochemical variability in the Iranian poppy (Papaver bracteatum Lindl.): A baseline for conservation and utilization in future breeding programmes
Författare: Qaderi, Ardashir; Omidi, Mansour; Pour-Aboughadareh, Alireza; Poczai, Péter; Shaghaghi, Javad; MehrAfarin, Ali; Nohooji, Majid Ghorbani; Etminan, Alireza
Upphovmannens organisation: Finnish Museum of Natural History
Embryophylo
Datum: 2019-04
Språk: eng
Sidantal: 11
Tillhör serie: Industrial Crops and Products
ISSN: 0926-6690
DOI: https://doi.org/10.1016/j.indcrop.2018.12.079
Permanenta länken (URI): http://hdl.handle.net/10138/323866
Abstrakt: In the present investigation, 72 accessions of the Iranian poppy (Papaver bracteatum Lindl.) were analyzed for genetic diversity and population structure using start codon targeted polymorphism (SCoT) and inter simple sequence repeat (ISSR) markers along with four important phytochemical traits to provide baseline knowledge for the Iranian poppy’s breeding and conservation plans. Twelve ISSR and thirteen SCoT primers generated a total of 98 and 186 fragments with a mean of 8.17 and 14.31 fragments per primer, respectively. Polymorphic information content for ISSR and SCoT primers ranged from 0.39 to 0.45 and 0.28 to 0.34, with the resolving power ranging from 21.61 to 3.97 and 13.08 to 28.02, respectively. Neighbour-joining (NJ) based clustering grouped 72 accessions into three main groups based on two markers studied (ISSR and SCoT) and the combined data (ISSR + SCoT), which associated with their eco-geographical regions. Population structure based analysis divided 72 accessions into 3 subpopulations using ISSR markers, when SCoT was used eight subpopulations were observed. However, when the combined data was used only three subpopulations were found, which corresponded to the grouping observed with the NJ method and these results were supported by principal coordinate analyses (PCoA). Phytochemical analysis revealed that plant capsule has higher total amounts of the alkaloids; thebaine, morphine and oripavine than stem tissues. Interestingly, for the geographical parameters, latitude showed a significant and positive correlation with thebaine extracted from both stem and capsules and the regression results confirmed these associations. Taken together, our results indicated that three populations Ploor, Eil-Teymoor and Anjomane due to their high contents of alkaloids like thebaine as well as the Taham population due to its high content of morphine and oripavine have a strong enough potency to be used in the pharmacy industry.
Subject: AFLP
ALKALOIDS
CODON TARGETED SCOT
DNA
GENETIC DIVERSITY
Gene-targeted markers
Genetic diversity
Germplasm
ISSR POLYMORPHISM
MARKERS
Morphine
POPULATION-STRUCTURE
Poppy
Population structure
SOMNIFERUM
Thebaine
1181 Ecology, evolutionary biology
4111 Agronomy
Referentgranskad: Ja
Licens: cc_by_nc_nd
Användningsbegränsning: openAccess
Parallelpublicerad version: acceptedVersion


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